Functions of isolated domains of methionyl-tRNA synthetase from an extreme thermophile, Thermus thermophilus HB8

D. Kohda, S. Yokoyama, T. Miyazawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Methionyl-tRNA synthetase (MetRS, 2 x 75 kDa) was purified to homogeneity from an extreme thermophile, Thermus thermophilus HB8. The polypeptide chain of MetRS was cleaved by limited digestion with trypsin into four domains: T1 (29 kDa), T2 (23 kDa), T3 (14.5 kDa), and T4 (7.5 kDa), which were aligned in that order. MetRS was also cleaved into similar fragments with a variety of other proteases. Domains T1, T2, T3, and T4 were isolated by column chromatography. 'Tandem domain' T1-T2 (56 kDa) is fully active in the aminoacylation of tRNA and is further cleaved with trypsin into domains T1 and T2. Domain T1 is the smallest aminoacylation unit so far reported. Domain T2 (enzymatically inactive) interacts with tRNA(f)(Met), as found by UV-induced cross-linking. Isolated domain T3 forms a dimer and is responsible for the dimer assembly of two protomers in MetRS. Domain T4 is a flexible tail of MetRS. These domains, in particular T1 and T2, will be important for detailed structure analyses in relation to aminoacylation activity.

Original languageEnglish
Pages (from-to)558-563
Number of pages6
JournalJournal of Biological Chemistry
Volume262
Issue number2
Publication statusPublished - Jan 1 1987

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Methionine-tRNA Ligase
Thermus thermophilus
Aminoacylation
Dimers
Trypsin
Transfer RNA Aminoacylation
RNA, Transfer, Met
Column chromatography
Protein Subunits
Transfer RNA
Chromatography
Digestion
Peptide Hydrolases
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functions of isolated domains of methionyl-tRNA synthetase from an extreme thermophile, Thermus thermophilus HB8. / Kohda, D.; Yokoyama, S.; Miyazawa, T.

In: Journal of Biological Chemistry, Vol. 262, No. 2, 01.01.1987, p. 558-563.

Research output: Contribution to journalArticle

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