Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice

Yukiko Tomioka, Masami Morimatsu, Keiko Amagai, Minako Kuramochi, Yuki Watanabe, Shigeto Kouda, Toshio Wada, Noritaka Kuboki, Etsuro Ono

Research output: Contribution to journalArticle

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Abstract

Nectin-1 is a Ca2+-independent Ig-like cell-cell adhesion molecule and an alphaherpesvirus receptor that binds to virion glycoprotein D by the first Ig-like domain. We have investigated the antiviral potentials of soluble forms of porcine nectin-1 to PRV infection by generating transgenicmice expressing different types of fusion protein. Previously, we reported that mice transgenic for a chimera that carried the entire ectodomain of porcine nectin-1 fused to the Fc portion of porcine IgG1 were more resistant than those transgenic for a chimera that carried the first Ig-like domain fused to the Fc portion. Recently, we generated transgenic mice expressing a fusion protein made of the first Ig-like domain fused to the Fc portion of human IgG1, and reported that they showed a microphthalmia. Here, two transgenic mouse lines expressing the fusion protein were challenged with PRV for comparing their resistances with those of transgenicmice expressing different types of fusion protein. Surprisingly, both transgenicmouse lines showed a high resistance to the viral infection, especially via the i.n. route. Significant resistance of the embryonic fibroblasts was also observed. Altogether, these findings indicated that the fusion protein consisting of the first Ig-like domain fused to the human Fc portion provided amarked resistance against PRV infection to the transgenicmice.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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Suid Herpesvirus 1
Virus Diseases
Transgenic Mice
Swine
Immunoglobulin G
Proteins
Microphthalmos
Cell Adhesion Molecules
Infection
Virion
Antiviral Agents
Glycoproteins
Fibroblasts
nectins
Immunoglobulin Domains

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice. / Tomioka, Yukiko; Morimatsu, Masami; Amagai, Keiko; Kuramochi, Minako; Watanabe, Yuki; Kouda, Shigeto; Wada, Toshio; Kuboki, Noritaka; Ono, Etsuro.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 53, No. 1, 01.01.2009, p. 8-15.

Research output: Contribution to journalArticle

Tomioka, Yukiko ; Morimatsu, Masami ; Amagai, Keiko ; Kuramochi, Minako ; Watanabe, Yuki ; Kouda, Shigeto ; Wada, Toshio ; Kuboki, Noritaka ; Ono, Etsuro. / Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice. In: MICROBIOLOGY and IMMUNOLOGY. 2009 ; Vol. 53, No. 1. pp. 8-15.
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