Gβγ counteracts Gαq signaling upon α1-adrenergic receptor stimulation

Motohiro Nishida, Shuichi Takagahara, Yoshiko Maruyama, Yoshiyuki Sugimoto, Taku Nagao, Hitoshi Kurose

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In rat neonatal myocytes, a constitutively active Gαq causes cellular injury and apoptosis. However, stimulation of the α1-adrenergic receptor, one of the Gq protein-coupled receptors, with phenylephrine for 48 h causes little cellular injury and apoptosis. Expression of the Gβγ-sequestering peptide βARK-ct increases the phenylephrine-induced cardiac injury, indicating that Gβγ released from Gq counteracts the Gαq-mediated cellular injury. Stimulation with phenylephrine activates extracellular signal-regulated kinase (ERK) and Akt, and activation is significantly blunted by βARK-ct. Inhibition of Akt by inhibitors of phosphatidylinositol 3-kinase increases the cellular injury induced by phenylephrine stimulation. In contrast to the inhibition of Akt, inhibition of ERK does not affect the phenylephrine-induced cardiac injury. These results suggest that Gβγ released from Gq upon α1-adrenergic receptor stimulation activates ERK and Akt. However, activation of Akt but not ERK plays an important role in the protection against the Gαq-induced cellular injury and apoptosis.

Original languageEnglish
Pages (from-to)995-1000
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number4
DOIs
Publication statusPublished - Mar 8 2002

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Phenylephrine
Adrenergic Receptors
Extracellular Signal-Regulated MAP Kinases
Wounds and Injuries
Apoptosis
Gq-G11 GTP-Binding Protein alpha Subunits
Chemical activation
Phosphatidylinositol 3-Kinase
Rats
Muscle Cells
Peptides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gβγ counteracts Gαq signaling upon α1-adrenergic receptor stimulation. / Nishida, Motohiro; Takagahara, Shuichi; Maruyama, Yoshiko; Sugimoto, Yoshiyuki; Nagao, Taku; Kurose, Hitoshi.

In: Biochemical and Biophysical Research Communications, Vol. 291, No. 4, 08.03.2002, p. 995-1000.

Research output: Contribution to journalArticle

Nishida, Motohiro ; Takagahara, Shuichi ; Maruyama, Yoshiko ; Sugimoto, Yoshiyuki ; Nagao, Taku ; Kurose, Hitoshi. / Gβγ counteracts Gαq signaling upon α1-adrenergic receptor stimulation. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 291, No. 4. pp. 995-1000.
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