G-CSF increases secretion of urokinase-type plasminogen activator by human lung cancer cells

Xin Hai Pei, Yoichi Nakanishi, Koichi Takayama, Fen Bai, Masayuki Kawasaki, Nobuyuki Hara

Research output: Contribution to journalArticle

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Abstract

We reported previously that granulocyte colony-stimulating factor (G- CSF) can promote the invasion of human lung cancer cell lines in vitro. However, the exact mechanism of its stimulatory effect on invasion remains to be elucidated. In the present study we mainly focused our attention on the components of the plasminogen activation system in human lung cancer cell lines, because of the central role that plasminogen activators play in regulating extracellular proteolysis. We showed that G-CSF induced a dose- dependent increase in the urokinase-type plasminogen activator (uPA) activity in the conditioned medium of a PC-9 lung cancer cell line. When the amounts of uPA activity were quantitated by densitometry, we found that even at a concentration of 0.01 μg/ml, G-CSF had a stimulatory effect on the uPA release, while high concentrations caused a 3.6-fold increase at a maximum concentration of 1 μg/ml. A Western blot analysis of the conditioned medium confirmed the findings observed in a zymographic analysis. The observed increase in uPA protein was paralleled by a significant increase in the uPA mRNA levels after treatment with G-CSF. However, our experiments failed to identify any alteration in the plasminogen activator inhibitor (PAI) secretion caused by G-CSF. In addition, we also found the expression of G- CSF receptor by PC-9 cells, suggesting the possible pathway activated by G- CSF.

Original languageEnglish
Pages (from-to)551-558
Number of pages8
JournalClinical and Experimental Metastasis
Volume16
Issue number6
DOIs
Publication statusPublished - Dec 1 1998

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Urokinase-Type Plasminogen Activator
Granulocyte Colony-Stimulating Factor
Lung Neoplasms
Conditioned Culture Medium
Cell Line
Granulocyte Colony-Stimulating Factor Receptors
Plasminogen Inactivators
Densitometry
Plasminogen Activators
Plasminogen
Proteolysis
Western Blotting
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

G-CSF increases secretion of urokinase-type plasminogen activator by human lung cancer cells. / Pei, Xin Hai; Nakanishi, Yoichi; Takayama, Koichi; Bai, Fen; Kawasaki, Masayuki; Hara, Nobuyuki.

In: Clinical and Experimental Metastasis, Vol. 16, No. 6, 01.12.1998, p. 551-558.

Research output: Contribution to journalArticle

Pei, Xin Hai ; Nakanishi, Yoichi ; Takayama, Koichi ; Bai, Fen ; Kawasaki, Masayuki ; Hara, Nobuyuki. / G-CSF increases secretion of urokinase-type plasminogen activator by human lung cancer cells. In: Clinical and Experimental Metastasis. 1998 ; Vol. 16, No. 6. pp. 551-558.
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