G protein γ7 expression as a new clinicopathological marker in patients with intrahepatic cholangiocarcinoma

Tohru Utsunomiya, Hiroshi Inoue, Ken Ichi Taguchi, Mitsuo Shimada, Keizo Sugimachi, Masaki Mori

Research output: Contribution to journalArticle

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Abstract

Hypothesis: The signal alterations mediated by small G proteins such as Ras, Rho, and Rac have been reported in several cancers. The human G protein γ7 (G-γ7) gene, which is down-regulated in various digestive organ cancers, was recently identified and cloned. Thus, the G-γ7-coupled heterotrimeric G proteins may also contribute to carcinogenesis in human cancers. Setting: University hospital and medical institute of bioregulation. Patients and Methods: The clinicopathological significance of G-γ7 expression in 18 patients with intrahepatic cholangiocarcinoma (IHCC) was examined. The tumor-nontumor ratio of G-γ7 expression was determined using reverse transcription polymerase chain reaction analysis. To visualize the localization of G-γ7, an immunohistochemical study was performed. Main Outcome Measure: Clinicopathological significance of G-γ7 expression in human IHCC. Results: Expression of G-γ7 messenger RNA was lower in tumor tissue than in the corresponding nontumor tissue in 17 (94%) of 18 patients with IHCC. The mean tumor-nontumor ratio was 0.54. Eleven patients with tumor-nontumor ratios less than 0.5 showed significantly poorer differentiated IHCC than 7 with tumor-nontumor ratios of 0.5 and greater (P<.01). Decreased expression of G-γ7 protein in the carcinoma tissue, especially in the poorly differentiated IHCC tissue, was confirmed using immunohistochemical staining. Conclusions: Reduced expression of G-γ7 is associated with the histological grade of IHCC and may therefore prove to be a useful marker for predicting the biological aggressiveness of human IHCC.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalArchives of Surgery
Volume137
Issue number2
DOIs
Publication statusPublished - Jan 1 2002

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Cholangiocarcinoma
GTP-Binding Proteins
Neoplasms
Heterotrimeric GTP-Binding Proteins
Monomeric GTP-Binding Proteins
Reverse Transcription
Carcinogenesis
Biomarkers
Outcome Assessment (Health Care)
Staining and Labeling
Carcinoma
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Surgery

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G protein γ7 expression as a new clinicopathological marker in patients with intrahepatic cholangiocarcinoma. / Utsunomiya, Tohru; Inoue, Hiroshi; Taguchi, Ken Ichi; Shimada, Mitsuo; Sugimachi, Keizo; Mori, Masaki.

In: Archives of Surgery, Vol. 137, No. 2, 01.01.2002, p. 181-185.

Research output: Contribution to journalArticle

Utsunomiya, Tohru ; Inoue, Hiroshi ; Taguchi, Ken Ichi ; Shimada, Mitsuo ; Sugimachi, Keizo ; Mori, Masaki. / G protein γ7 expression as a new clinicopathological marker in patients with intrahepatic cholangiocarcinoma. In: Archives of Surgery. 2002 ; Vol. 137, No. 2. pp. 181-185.
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