G-proteins and adenylyl cyclase in ovarian granulosa cells of amago salmon (Oncorhynchus rhodurus)

Mita Masatoshi, Michiyasu Yoshikuni, Nagahama Yoshitaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The involvement of guanine nucleotide-binding regulatory proteins (G-proteins) and adenylyl cyclase in the gonadotropin stimulation of cAMP was investigated using crude membrane fractions from granulosa cells of amago salmon (Oncorhynchus rhodurus) postvitellogenic ovarian follicles. Although cholera toxin-catalyzed ADP ribosylation occurred in 45- and 50-kDa proteins, only the former was recognized by an antibody against the α-subunit of Gs. With pertussis toxin, only the 41-kDa protein was ADP- ribosylated. This 41-kDa protein was recognized by an antibody against the α-subunit of Gi. Partially purified chum salmon gonadotropin (SGA) stimulated adenylyl cyclase activity in crude membrane preparations of granulosa cells only in the presence of pertussis toxin in the incubation medium. Adenosine inhibited adenylyl cyclase in the presence of GTP and pertussis toxin reversed it. Unlike SGA, forskolin, which acts upon adenylyl cyclase without G-protein interaction, markedly stimulated adenylyl cyclase activity in the absence of pertussis toxin. These results provide evidence that both stimulatory (Gs) and inhibitory (Gi) regulation by adenylyl cyclase operates in the granulosa cells of amago salmon postvitellogenic ovarian follicles. It is possible that, although a stimulatory receptor interacts with Gs, its activity is influenced by the functional state of Gi.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume105
Issue number1
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

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Oncorhynchus
Guanine Nucleotides
Granulosa Cells
Salmon
GTP-Binding Proteins
Adenylyl Cyclases
Carrier Proteins
Pertussis Toxin
Proteins
Ovarian Follicle
Adenosine Diphosphate
Oncorhynchus keta
Membranes
Antibodies
Cholera Toxin
Colforsin
Guanosine Triphosphate
Gonadotropins
Adenosine

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

G-proteins and adenylyl cyclase in ovarian granulosa cells of amago salmon (Oncorhynchus rhodurus). / Masatoshi, Mita; Yoshikuni, Michiyasu; Yoshitaka, Nagahama.

In: Molecular and Cellular Endocrinology, Vol. 105, No. 1, 01.01.1994, p. 83-88.

Research output: Contribution to journalArticle

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