GABA-induced response in spiral ganglion cells acutely isolated from guinea pig cochlea

Takashi Nakagawa, Michiko Yamashita, Kazutaka Hisashi, Shin Ich Usami, Yasuhiro Kakazu, Shumei Shibata, Torahiko Nakashima, Koji Koike, Kazuhiko Kubo, Shizuo Komune

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The physiological and pharmacological properties of γ-aminobutyric acid (GABA)-induced responses were investigated in acutely isolated spiral ganglion cells (SGCs) of guinea pig by using either a nystatin-perforated patch recording configuration or a conventional whole-cell patch recording mode combined with rapid drug application. GABA and GABAA subtype receptor agonist, muscimol, induced inward currents in a concentration-dependent manner in 74% of all cells. The current-voltage relationship for the GABA response indicated the GABA-induced current in SGCs is carried by Cl-. Bicuculline (BIC), strychnine (STR), and picrotoxin (PTX) suppressed the GABA response in a concentration-dependent manner. BIC and STR, and PTX blocked the GABA response in a competitive manner and in a non-competitive manner, respectively. For inorganic antagonists, Cd2+ and Ni2+ also inhibited the GABA response. On the other hand, Zn2+ failed to suppress the GABA response in SGCs. An antibiotic, benzylpenicillin, suppressed the GABA response. The GABA response was augmented by both a barbiturate derivative, pentobarbital (PB), and a benzodiazepine derivative, diazepam. The results suggest clearly that the physiological and pharmacological characteristics of GABAA receptor on acutely isolated guinea pig SGCs are quite similar to the common GABAA receptor found in other sensory ganglion cells.

Original languageEnglish
Pages (from-to)396-403
Number of pages8
JournalNeuroscience Research
Volume53
Issue number4
DOIs
Publication statusPublished - Dec 1 2005

Fingerprint

Spiral Ganglion
Cochlea
gamma-Aminobutyric Acid
Guinea Pigs
Strychnine
Picrotoxin
Bicuculline
GABA-A Receptors
Pharmacology
Sensory Ganglia
GABA-A Receptor Agonists
Aminobutyrates
Nystatin
Muscimol
Penicillin G
Patch-Clamp Techniques
Pentobarbital
Diazepam
Benzodiazepines

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Nakagawa, T., Yamashita, M., Hisashi, K., Usami, S. I., Kakazu, Y., Shibata, S., ... Komune, S. (2005). GABA-induced response in spiral ganglion cells acutely isolated from guinea pig cochlea. Neuroscience Research, 53(4), 396-403. https://doi.org/10.1016/j.neures.2005.08.011

GABA-induced response in spiral ganglion cells acutely isolated from guinea pig cochlea. / Nakagawa, Takashi; Yamashita, Michiko; Hisashi, Kazutaka; Usami, Shin Ich; Kakazu, Yasuhiro; Shibata, Shumei; Nakashima, Torahiko; Koike, Koji; Kubo, Kazuhiko; Komune, Shizuo.

In: Neuroscience Research, Vol. 53, No. 4, 01.12.2005, p. 396-403.

Research output: Contribution to journalArticle

Nakagawa, T, Yamashita, M, Hisashi, K, Usami, SI, Kakazu, Y, Shibata, S, Nakashima, T, Koike, K, Kubo, K & Komune, S 2005, 'GABA-induced response in spiral ganglion cells acutely isolated from guinea pig cochlea', Neuroscience Research, vol. 53, no. 4, pp. 396-403. https://doi.org/10.1016/j.neures.2005.08.011
Nakagawa, Takashi ; Yamashita, Michiko ; Hisashi, Kazutaka ; Usami, Shin Ich ; Kakazu, Yasuhiro ; Shibata, Shumei ; Nakashima, Torahiko ; Koike, Koji ; Kubo, Kazuhiko ; Komune, Shizuo. / GABA-induced response in spiral ganglion cells acutely isolated from guinea pig cochlea. In: Neuroscience Research. 2005 ; Vol. 53, No. 4. pp. 396-403.
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