Abstract
Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 398-405 |
| Number of pages | 8 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 72 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 13 2008 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Galactinol synthase gene of Coptis japonica is involved in berberine tolerance. / Takanashi, Kojiro; Shitan, Nobukazu; Sugiyama, Akifumi; Kamimoto, Yoshihisa; Hamamoto, Masafumi; Iwaki, Tomoko; Takegawa, Kaoru; Yazaki, Kazufumi.
In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 2, 13.03.2008, p. 398-405.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Galactinol synthase gene of Coptis japonica is involved in berberine tolerance
AU - Takanashi, Kojiro
AU - Shitan, Nobukazu
AU - Sugiyama, Akifumi
AU - Kamimoto, Yoshihisa
AU - Hamamoto, Masafumi
AU - Iwaki, Tomoko
AU - Takegawa, Kaoru
AU - Yazaki, Kazufumi
PY - 2008/3/13
Y1 - 2008/3/13
N2 - Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.
AB - Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.
UR - http://www.scopus.com/inward/record.url?scp=40449083271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40449083271&partnerID=8YFLogxK
U2 - 10.1271/bbb.70495
DO - 10.1271/bbb.70495
M3 - Article
C2 - 18256493
AN - SCOPUS:40449083271
VL - 72
SP - 398
EP - 405
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -