Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus

K. Kajitani, H. Nomaru, M. Ifuku, N. Yutsudo, Y. Dan, T. Miura, D. Tsuchimoto, K. Sakumi, T. Kadoya, H. Horie, F. Poirier, M. Noda, Y. Nakabeppu

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Abstract

We examined the expression of galectin-1, an endogenous lectin with one carbohydrate-binding domain, in the adult mouse hippocampus after systemic kainate administration. We found that the expression of galectin-1 was remarkably increased in activated astrocytes of the CA3 subregion and dentate gyrus of the hippocampus, and in nestin-positive neural progenitors in the dentate gyrus. Quantitative reverse transcription PCR (RT-PCR) analysis revealed that the galectin-1 mRNA level in hippocampus began to increase 1 day after kainate administration and that a 13-fold increase was attained within 3 days. Western blotting analysis confirmed that the level of galectin-1 protein increased to more than three-fold a week after the exposure. We showed that isolated astrocytes express and secrete galectin-1. To clarify the significance of the increased expression of galectin-1 in hippocampus, we compared the levels of hippocampal cell proliferation in galectin-1 knockout and wild-type mice after saline or kainate administration. The number of 5-bromo-2′-deoxyuridine (BrdU)-positive cells detected in the subgranular zone (SGZ) of galectin-1 knockout mice decreased to 62% with saline, and to 52% with kainate, as compared with the number seen in the wild-type mice. Most of the BrdU-positive cells in SGZ expressed doublecortin and neuron-specific nuclear protein, indicating that they are immature neurons. We therefore concluded that galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the hippocampus.

Original languageEnglish
Pages (from-to)417-427
Number of pages11
JournalCell Death and Differentiation
Volume16
Issue number3
DOIs
Publication statusPublished - Jan 1 2009

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Galectin 1
Kainic Acid
Dentate Gyrus
Hippocampus
Bromodeoxyuridine
Knockout Mice
Astrocytes
Neurons
Nestin
Parahippocampal Gyrus
Nuclear Proteins
Lectins
Reverse Transcription
Western Blotting
Carbohydrates
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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Galectin-1 promotes basal and kainate-induced proliferation of neural progenitors in the dentate gyrus of adult mouse hippocampus. / Kajitani, K.; Nomaru, H.; Ifuku, M.; Yutsudo, N.; Dan, Y.; Miura, T.; Tsuchimoto, D.; Sakumi, K.; Kadoya, T.; Horie, H.; Poirier, F.; Noda, M.; Nakabeppu, Y.

In: Cell Death and Differentiation, Vol. 16, No. 3, 01.01.2009, p. 417-427.

Research output: Contribution to journalArticle

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