GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein

Yasuyuki Fujita, Takuya Sasaki, Keishi Araki, Kazuo Takahashi, Katsunori Imazumi, Masaki Kato, Yoshiharu Matsuura, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Rabphilin-3A is a putative target protein for Rab3A, a small GTP-binding protein particularly implicated in neurotransmitter release. Rabphilin-3A interacts more preferentially with GTP-Rab3A than with GDP-Rab3A. Moreover, Rabphilin-3A shows a weak activity to stimulate the GTPase activity of Rab3A and a strong activity to inhibit the Rab3A GTPase-activating protein (GAP)-stimulated GTPase activity of Rab3A. Here, we show that Rabphilin-3A has another activity to stimulate the GDP/GTP exchange reaction of Rab3A. Rabphifin-3A may keep Rab3A continuously in the GTP-bound form by converting again GDP-Rab3A, which may be converted from GTP-Rab3A by Rab3A GAP, to GTP-Rab3A, until the function of Rab3A is accomplished.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalFEBS Letters
Volume353
Issue number1
DOIs
Publication statusPublished - Oct 10 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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