GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein

Yasuyuki Fujita, Takuya Sasaki, Keishi Araki, Kazuo Takahashi, Katsunori Imazumi, Masaki Kato, Yoshiharu Matsuura, Yoshimi Takai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rabphilin-3A is a putative target protein for Rab3A, a small GTP-binding protein particularly implicated in neurotransmitter release. Rabphilin-3A interacts more preferentially with GTP-Rab3A than with GDP-Rab3A. Moreover, Rabphilin-3A shows a weak activity to stimulate the GTPase activity of Rab3A and a strong activity to inhibit the Rab3A GTPase-activating protein (GAP)-stimulated GTPase activity of Rab3A. Here, we show that Rabphilin-3A has another activity to stimulate the GDP/GTP exchange reaction of Rab3A. Rabphifin-3A may keep Rab3A continuously in the GTP-bound form by converting again GDP-Rab3A, which may be converted from GTP-Rab3A by Rab3A GAP, to GTP-Rab3A, until the function of Rab3A is accomplished.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalFEBS Letters
Volume353
Issue number1
DOIs
Publication statusPublished - Oct 10 1994

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rab3A GTP-Binding Protein
Guanosine Triphosphate
GTP-Binding Proteins
GTPase-Activating Proteins
GTP Phosphohydrolases
Neurotransmitter Agents
rabphilin-3A

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein. / Fujita, Yasuyuki; Sasaki, Takuya; Araki, Keishi; Takahashi, Kazuo; Imazumi, Katsunori; Kato, Masaki; Matsuura, Yoshiharu; Takai, Yoshimi.

In: FEBS Letters, Vol. 353, No. 1, 10.10.1994, p. 67-70.

Research output: Contribution to journalArticle

Fujita, Y, Sasaki, T, Araki, K, Takahashi, K, Imazumi, K, Kato, M, Matsuura, Y & Takai, Y 1994, 'GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein', FEBS Letters, vol. 353, no. 1, pp. 67-70. https://doi.org/10.1016/0014-5793(94)01015-3
Fujita, Yasuyuki ; Sasaki, Takuya ; Araki, Keishi ; Takahashi, Kazuo ; Imazumi, Katsunori ; Kato, Masaki ; Matsuura, Yoshiharu ; Takai, Yoshimi. / GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein. In: FEBS Letters. 1994 ; Vol. 353, No. 1. pp. 67-70.
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