GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein

Yasuyuki Fujita, Takuya Sasaki, Keishi Araki, Kazuo Takahashi, Katsunori Imazumi, Masaki Kato, Yoshiharu Matsuura, Yoshimi Takai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rabphilin-3A is a putative target protein for Rab3A, a small GTP-binding protein particularly implicated in neurotransmitter release. Rabphilin-3A interacts more preferentially with GTP-Rab3A than with GDP-Rab3A. Moreover, Rabphilin-3A shows a weak activity to stimulate the GTPase activity of Rab3A and a strong activity to inhibit the Rab3A GTPase-activating protein (GAP)-stimulated GTPase activity of Rab3A. Here, we show that Rabphilin-3A has another activity to stimulate the GDP/GTP exchange reaction of Rab3A. Rabphifin-3A may keep Rab3A continuously in the GTP-bound form by converting again GDP-Rab3A, which may be converted from GTP-Rab3A by Rab3A GAP, to GTP-Rab3A, until the function of Rab3A is accomplished.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalFEBS Letters
Volume353
Issue number1
DOIs
Publication statusPublished - Oct 10 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein'. Together they form a unique fingerprint.

  • Cite this

    Fujita, Y., Sasaki, T., Araki, K., Takahashi, K., Imazumi, K., Kato, M., Matsuura, Y., & Takai, Y. (1994). GDP/GTP exchange reaction-stimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein. FEBS Letters, 353(1), 67-70. https://doi.org/10.1016/0014-5793(94)01015-3