Gene delivery system based on highly specific recognition of surface-vimentin with N-acetylglucosamine immobilized polyethylenimine

Sun Jung Kim, Hirohiko Ise, Mitsuaki Goto, Kenta Komura, Chong Su Cho, Toshihiro Akaike

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Gene and drug-delivery systems that use immobilization of carbohydrates are useful for the specific targeting of lectin-expressing tissues. Here, we report that N-acetylglucosamine (GlcNAc) with polyethylenimine (GlcNAc-PEI) specifically interacted with vimentin-expressing cells such as 293FT and HeLa cells. Recently, the intermediate filaments vimentin and desmin have been reported to have GlcNAc-binding lectin-like properties on the cell surface. Therefore, GlcNAc-conjugated agents can be targeted to vimentin- and desmin-expressing cells and tissues. Vimentin-expressing 293FT and HeLa cells were efficiently transfected with green fluorescent protein and luciferase genes by using GlcNAc-PEI; the expression of these genes in vimentin-knockdown cells were low. Confocal microscopic analysis showed that GlcNAc-PEI complexes interacted with vimentin on the cell surface of HeLa cells. These results demonstrate that GlcNAc-PEI/DNA complexes were specifically taken up by 293FT and HeLa cells via vimentin. We suggest that this gene-delivery system could be used to target various vimentin-expressing cells such as fibroblasts and tumor cells.

Original languageEnglish
Pages (from-to)3471-3480
Number of pages10
JournalBiomaterials
Volume32
Issue number13
DOIs
Publication statusPublished - May 1 2011
Externally publishedYes

Fingerprint

Polyethyleneimine
Gene Transfer Techniques
Acetylglucosamine
Vimentin
Genes
HeLa Cells
Tissue
Desmin
Fibroblasts
Carbohydrates
Lectins
Tumors
DNA
Cells
Proteins
Intermediate Filaments
Drug Delivery Systems
Green Fluorescent Proteins
Luciferases
Immobilization

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Gene delivery system based on highly specific recognition of surface-vimentin with N-acetylglucosamine immobilized polyethylenimine. / Kim, Sun Jung; Ise, Hirohiko; Goto, Mitsuaki; Komura, Kenta; Cho, Chong Su; Akaike, Toshihiro.

In: Biomaterials, Vol. 32, No. 13, 01.05.2011, p. 3471-3480.

Research output: Contribution to journalArticle

Kim, Sun Jung ; Ise, Hirohiko ; Goto, Mitsuaki ; Komura, Kenta ; Cho, Chong Su ; Akaike, Toshihiro. / Gene delivery system based on highly specific recognition of surface-vimentin with N-acetylglucosamine immobilized polyethylenimine. In: Biomaterials. 2011 ; Vol. 32, No. 13. pp. 3471-3480.
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