Gene delivery system responding to the intracellular signal

Kenji Kawamura, Yoshiki Katayama

    Research output: Contribution to journalReview articlepeer-review

    1 Citation (Scopus)

    Abstract

    We designed a peptide-polymer conjugate as a novel gene carrier, which could control gene-expression responding to intracellular cAMP-dependent protein kinase (PKA) or caspase-3 signal. Each polymer containing a substrate peptide (ALRRASLG for PKA or GGGDEVD GGRKKRRQRRRPPQ for caspase-3) could form a tight complex with plasmid DNA via electrostatic interaction, and in this state, the gene expression was totally suppressed. Once the grafted peptides become phosphorylated or hydrolyzed by the appropriate enzyme, the net charge of the peptide changes from cationic to neutral or anionic, respectively, and the polymer then releases the DNA, such that expression of the foreign gene should become activated. We expect that these systems can be applied to the novel highly cell specific gene delivery strategy.

    Original languageEnglish
    Pages (from-to)265-270
    Number of pages6
    JournalNippon rinsho. Japanese journal of clinical medicine
    Volume64
    Issue number2
    Publication statusPublished - Jan 1 2006

    All Science Journal Classification (ASJC) codes

    • Medicine(all)

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