Gene delivery system responding to the intracellular signal

Kenji Kawamura, Yoshiki Katayama

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

We designed a peptide-polymer conjugate as a novel gene carrier, which could control gene-expression responding to intracellular cAMP-dependent protein kinase (PKA) or caspase-3 signal. Each polymer containing a substrate peptide (ALRRASLG for PKA or GGGDEVD GGRKKRRQRRRPPQ for caspase-3) could form a tight complex with plasmid DNA via electrostatic interaction, and in this state, the gene expression was totally suppressed. Once the grafted peptides become phosphorylated or hydrolyzed by the appropriate enzyme, the net charge of the peptide changes from cationic to neutral or anionic, respectively, and the polymer then releases the DNA, such that expression of the foreign gene should become activated. We expect that these systems can be applied to the novel highly cell specific gene delivery strategy.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalNippon rinsho. Japanese journal of clinical medicine
Volume64
Issue number2
Publication statusPublished - Jan 1 2006

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Gene Transfer Techniques
Polymers
Peptides
Gene Expression
Caspase 3
DNA
Cyclic AMP-Dependent Protein Kinases
Static Electricity
Protein Kinases
Genes
Plasmids
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Gene delivery system responding to the intracellular signal. / Kawamura, Kenji; Katayama, Yoshiki.

In: Nippon rinsho. Japanese journal of clinical medicine, Vol. 64, No. 2, 01.01.2006, p. 265-270.

Research output: Contribution to journalReview article

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