Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate

Hideaki Shibata, Naoki Kanayama, Tohru Takarada, Hideo Nakashita, Toshiaki Kudo, Mizuo Maeda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a method for separating a mixture of DNAs having a single-base difference by using weak affinity capillary electrophoresis (WACE). DNA-poly(ethylene glycol) (PEG) conjugate was synthesized as a separation medium of WACE. A DNA unit of the conjugate was designed to be complementary to the wild-type sample DNA and was coupled with a PEG unit for retardation of the electrophoretic migration in the capillary to act as an affinity-probe against wild-type sample DNA. The DNA-PEG conjugate properly worked as a separation medium, and a mixture of 60 mer DNA and its single-base mutant was successfully separated by the present method.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan - 55th SPSJ Annual Meeting
Pages2061
Number of pages1
Volume55
Edition1
Publication statusPublished - 2006
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

Fingerprint

Capillary electrophoresis
Polyethylene glycols
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Shibata, H., Kanayama, N., Takarada, T., Nakashita, H., Kudo, T., & Maeda, M. (2006). Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate. In Polymer Preprints, Japan - 55th SPSJ Annual Meeting (1 ed., Vol. 55, pp. 2061)

Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate. / Shibata, Hideaki; Kanayama, Naoki; Takarada, Tohru; Nakashita, Hideo; Kudo, Toshiaki; Maeda, Mizuo.

Polymer Preprints, Japan - 55th SPSJ Annual Meeting. Vol. 55 1. ed. 2006. p. 2061.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shibata, H, Kanayama, N, Takarada, T, Nakashita, H, Kudo, T & Maeda, M 2006, Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate. in Polymer Preprints, Japan - 55th SPSJ Annual Meeting. 1 edn, vol. 55, pp. 2061, 55th SPSJ Annual Meeting, Nagoya, Japan, 5/24/06.
Shibata H, Kanayama N, Takarada T, Nakashita H, Kudo T, Maeda M. Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate. In Polymer Preprints, Japan - 55th SPSJ Annual Meeting. 1 ed. Vol. 55. 2006. p. 2061
Shibata, Hideaki ; Kanayama, Naoki ; Takarada, Tohru ; Nakashita, Hideo ; Kudo, Toshiaki ; Maeda, Mizuo. / Gene diagnosis by weak affinity capillary electrophoresis using a DNA-PEG conjugate. Polymer Preprints, Japan - 55th SPSJ Annual Meeting. Vol. 55 1. ed. 2006. pp. 2061
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