TY - CHAP
T1 - Gene expression analysis of CCN protein in bone under mechanical stress
AU - Takano-Yamamoto, Teruko
AU - Fukunaga, Tomohiro
AU - Takeshita, Nobuo
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2017.
PY - 2017
Y1 - 2017
N2 - To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.
AB - To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.
UR - http://www.scopus.com/inward/record.url?scp=84991579870&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991579870&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6430-7_26
DO - 10.1007/978-1-4939-6430-7_26
M3 - Chapter
C2 - 27734385
AN - SCOPUS:84991579870
T3 - Methods in Molecular Biology
SP - 283
EP - 308
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -