TY - JOUR
T1 - Gene expression and functional abnormalities in XX/Sry Leydig cells
AU - Yanai, Shogo
AU - Baba, Takashi
AU - Inui, Kai
AU - Miyabayashi, Kanako
AU - Han, Soyun
AU - Inoue, Miki
AU - Takahashi, Fumiya
AU - Kanai, Yoshiakira
AU - Ohkawa, Yasuyuki
AU - Choi, Man Ho
AU - Morohashi, Ken ichirou
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers JP17H06427 (K.-i.M.), JP16H05142 (K.-i.M.), JP16K08593 (T.B.), and JP19J12133 (S.Y.), Takeda Science Foundation (T.B.), and The Shin-Nihon Foundation of Advanced Medical Research (T.B.). This work was technically supported by the Research Support Center, Graduate School of Medical Sciences, Kyushu University. We are profoundly thankful to Prof. Mikita Suyama (Kyushu University, Japan) for technical support in the NGS data processing, and Profs Hitoshi Okamura and Masao Doi (Kyoto University, Japan) for kindly providing the HSD3B6 antibody.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - The SRY gene induces testis development even in XX individuals. However, XX/Sry testes fail to produce mature sperm, due to the absence of Y chromosome carrying genes essential for spermatogenesis. XX/Sry Sertoli cells show abnormalities in the production of lactate and cholesterol required for germ cell development. Leydig cells are essential for male functions through testosterone production. However, whether XX/Sry adult Leydig cells (XX/Sry ALCs) function normally remains unclear. In this study, the transcriptomes from XY and XX/Sry ALCs demonstrated that immediate early and cholesterogenic gene expressions differed between these cells. Interestingly, cholesterogenic genes were upregulated in XX/Sry ALCs, although downregulated in XX/Sry Sertoli cells. Among the steroidogenic enzymes, CYP17A1 mediates steroid 17α-hydroxylation and 17,20-lyase reaction, necessary for testosterone production. In XX/Sry ALCs, the latter reaction was selectively decreased. The defects in XX/Sry ALCs, together with those in the germ and Sertoli cells, might explain the infertility of XX/Sry testes.
AB - The SRY gene induces testis development even in XX individuals. However, XX/Sry testes fail to produce mature sperm, due to the absence of Y chromosome carrying genes essential for spermatogenesis. XX/Sry Sertoli cells show abnormalities in the production of lactate and cholesterol required for germ cell development. Leydig cells are essential for male functions through testosterone production. However, whether XX/Sry adult Leydig cells (XX/Sry ALCs) function normally remains unclear. In this study, the transcriptomes from XY and XX/Sry ALCs demonstrated that immediate early and cholesterogenic gene expressions differed between these cells. Interestingly, cholesterogenic genes were upregulated in XX/Sry ALCs, although downregulated in XX/Sry Sertoli cells. Among the steroidogenic enzymes, CYP17A1 mediates steroid 17α-hydroxylation and 17,20-lyase reaction, necessary for testosterone production. In XX/Sry ALCs, the latter reaction was selectively decreased. The defects in XX/Sry ALCs, together with those in the germ and Sertoli cells, might explain the infertility of XX/Sry testes.
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U2 - 10.1038/s41598-020-80741-z
DO - 10.1038/s41598-020-80741-z
M3 - Article
C2 - 33436964
AN - SCOPUS:85099376026
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 719
ER -