Gene Expression in Blastocysts Following Direct Injection of DNA into Testis

Shyoso Ogawa, Katsuhiko Hayashi, Norihiro Tada, Masahiro Sato, Takashi Kurihara, Makoto Iwaya

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Liposome-encapsulated plasmid pMT-neo/MT-β-gal DNA containing a neomycin resistance gene and a E. coli β-galactosidase gene was injected through a needle into mature mouse testes bilaterally 3 times at 4-day intervals. Eighty percent of blastocysts derived from eggs fertilized by spermatozoa of male mice injected with the DNA exhibited expression of β-galactosidase gene and the presence of pMT-neo/MT-β-gal DNA. Thus, direct injection of foreign DNA into the testis will provide a novel and convenient tool for production of transgenic animals.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalJournal of Reproduction and Development
Volume41
Issue number4
DOIs
Publication statusPublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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