Gene expression levels and immunolocalization of organic ion transporters in the human kidney

Hideyuki Motohashi, Yuji Sakurai, Hideyuki Saito, Satohiro Masuda, Yumiko Urakami, Maki Goto, Atsushi Fukatsu, Osamu Ogawa, Ken Ichi Inui

Research output: Contribution to journalArticle

387 Citations (Scopus)

Abstract

Renal excretion of organic anions and cations is mediated by the organic ion transporter family (SLC22A). In this study, the mRNA levels of the organic ion transporters were quantified by real-time PCR in normal parts of renal tissues from seven nephrectomized patients with renal cell carcinoma, and the distributions and localization of human (h)OAT1, hOAT3, and hOCT2 proteins were investigated by immunohistochemical analyses in the human kidney. The expression level of hOAT3 mRNA was the highest among the organic ion transporter family, followed by that of hOAT1 mRNA. The hOCT2 mRNA level was the highest in the human OCT family, and the level of hOCTN2 mRNA was higher than that of hOCTN1. hOCT1 mRNA showed the lowest level of expression in organic ion transporter family. hOAT1, hOAT3, and hOCT2 proteins were detected in crude membranes from the kidney of all patients by Western blot analyses, whereas hOCT1 protein could not be detected. Immunohistochemical analyses showed that both hOAT1 and hOAT3 were localized to the basolateral membrane of the proximal tubules in the cortex, and hOCT2 was localized to the basolateral membrane of the proximal tubules in both the cortex and medullary ray. Immunohistochemical analyses of serial sections indicated that hOAT1, hOAT3, and hOCT2 were coexpressed in a portion of the proximal tubules. These results suggest that hOAT1, hOAT3, and hOCT2 play predominant roles in the transport of organic ions across the basolateral membrane of human proximal tubules.

Original languageEnglish
Pages (from-to)866-874
Number of pages9
JournalJournal of the American Society of Nephrology : JASN
Volume13
Issue number4
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Ions
Kidney
Gene Expression
Messenger RNA
Membranes
Proteins
Ion Transport
Renal Cell Carcinoma
Anions
Cations
Real-Time Polymerase Chain Reaction
Western Blotting

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Motohashi, H., Sakurai, Y., Saito, H., Masuda, S., Urakami, Y., Goto, M., ... Inui, K. I. (2002). Gene expression levels and immunolocalization of organic ion transporters in the human kidney. Journal of the American Society of Nephrology : JASN, 13(4), 866-874.

Gene expression levels and immunolocalization of organic ion transporters in the human kidney. / Motohashi, Hideyuki; Sakurai, Yuji; Saito, Hideyuki; Masuda, Satohiro; Urakami, Yumiko; Goto, Maki; Fukatsu, Atsushi; Ogawa, Osamu; Inui, Ken Ichi.

In: Journal of the American Society of Nephrology : JASN, Vol. 13, No. 4, 2002, p. 866-874.

Research output: Contribution to journalArticle

Motohashi, H, Sakurai, Y, Saito, H, Masuda, S, Urakami, Y, Goto, M, Fukatsu, A, Ogawa, O & Inui, KI 2002, 'Gene expression levels and immunolocalization of organic ion transporters in the human kidney', Journal of the American Society of Nephrology : JASN, vol. 13, no. 4, pp. 866-874.
Motohashi, Hideyuki ; Sakurai, Yuji ; Saito, Hideyuki ; Masuda, Satohiro ; Urakami, Yumiko ; Goto, Maki ; Fukatsu, Atsushi ; Ogawa, Osamu ; Inui, Ken Ichi. / Gene expression levels and immunolocalization of organic ion transporters in the human kidney. In: Journal of the American Society of Nephrology : JASN. 2002 ; Vol. 13, No. 4. pp. 866-874.
@article{0b208d1fc59a471fb94be3ef148cbb19,
title = "Gene expression levels and immunolocalization of organic ion transporters in the human kidney",
abstract = "Renal excretion of organic anions and cations is mediated by the organic ion transporter family (SLC22A). In this study, the mRNA levels of the organic ion transporters were quantified by real-time PCR in normal parts of renal tissues from seven nephrectomized patients with renal cell carcinoma, and the distributions and localization of human (h)OAT1, hOAT3, and hOCT2 proteins were investigated by immunohistochemical analyses in the human kidney. The expression level of hOAT3 mRNA was the highest among the organic ion transporter family, followed by that of hOAT1 mRNA. The hOCT2 mRNA level was the highest in the human OCT family, and the level of hOCTN2 mRNA was higher than that of hOCTN1. hOCT1 mRNA showed the lowest level of expression in organic ion transporter family. hOAT1, hOAT3, and hOCT2 proteins were detected in crude membranes from the kidney of all patients by Western blot analyses, whereas hOCT1 protein could not be detected. Immunohistochemical analyses showed that both hOAT1 and hOAT3 were localized to the basolateral membrane of the proximal tubules in the cortex, and hOCT2 was localized to the basolateral membrane of the proximal tubules in both the cortex and medullary ray. Immunohistochemical analyses of serial sections indicated that hOAT1, hOAT3, and hOCT2 were coexpressed in a portion of the proximal tubules. These results suggest that hOAT1, hOAT3, and hOCT2 play predominant roles in the transport of organic ions across the basolateral membrane of human proximal tubules.",
author = "Hideyuki Motohashi and Yuji Sakurai and Hideyuki Saito and Satohiro Masuda and Yumiko Urakami and Maki Goto and Atsushi Fukatsu and Osamu Ogawa and Inui, {Ken Ichi}",
year = "2002",
language = "English",
volume = "13",
pages = "866--874",
journal = "Journal of the American Society of Nephrology : JASN",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "4",

}

TY - JOUR

T1 - Gene expression levels and immunolocalization of organic ion transporters in the human kidney

AU - Motohashi, Hideyuki

AU - Sakurai, Yuji

AU - Saito, Hideyuki

AU - Masuda, Satohiro

AU - Urakami, Yumiko

AU - Goto, Maki

AU - Fukatsu, Atsushi

AU - Ogawa, Osamu

AU - Inui, Ken Ichi

PY - 2002

Y1 - 2002

N2 - Renal excretion of organic anions and cations is mediated by the organic ion transporter family (SLC22A). In this study, the mRNA levels of the organic ion transporters were quantified by real-time PCR in normal parts of renal tissues from seven nephrectomized patients with renal cell carcinoma, and the distributions and localization of human (h)OAT1, hOAT3, and hOCT2 proteins were investigated by immunohistochemical analyses in the human kidney. The expression level of hOAT3 mRNA was the highest among the organic ion transporter family, followed by that of hOAT1 mRNA. The hOCT2 mRNA level was the highest in the human OCT family, and the level of hOCTN2 mRNA was higher than that of hOCTN1. hOCT1 mRNA showed the lowest level of expression in organic ion transporter family. hOAT1, hOAT3, and hOCT2 proteins were detected in crude membranes from the kidney of all patients by Western blot analyses, whereas hOCT1 protein could not be detected. Immunohistochemical analyses showed that both hOAT1 and hOAT3 were localized to the basolateral membrane of the proximal tubules in the cortex, and hOCT2 was localized to the basolateral membrane of the proximal tubules in both the cortex and medullary ray. Immunohistochemical analyses of serial sections indicated that hOAT1, hOAT3, and hOCT2 were coexpressed in a portion of the proximal tubules. These results suggest that hOAT1, hOAT3, and hOCT2 play predominant roles in the transport of organic ions across the basolateral membrane of human proximal tubules.

AB - Renal excretion of organic anions and cations is mediated by the organic ion transporter family (SLC22A). In this study, the mRNA levels of the organic ion transporters were quantified by real-time PCR in normal parts of renal tissues from seven nephrectomized patients with renal cell carcinoma, and the distributions and localization of human (h)OAT1, hOAT3, and hOCT2 proteins were investigated by immunohistochemical analyses in the human kidney. The expression level of hOAT3 mRNA was the highest among the organic ion transporter family, followed by that of hOAT1 mRNA. The hOCT2 mRNA level was the highest in the human OCT family, and the level of hOCTN2 mRNA was higher than that of hOCTN1. hOCT1 mRNA showed the lowest level of expression in organic ion transporter family. hOAT1, hOAT3, and hOCT2 proteins were detected in crude membranes from the kidney of all patients by Western blot analyses, whereas hOCT1 protein could not be detected. Immunohistochemical analyses showed that both hOAT1 and hOAT3 were localized to the basolateral membrane of the proximal tubules in the cortex, and hOCT2 was localized to the basolateral membrane of the proximal tubules in both the cortex and medullary ray. Immunohistochemical analyses of serial sections indicated that hOAT1, hOAT3, and hOCT2 were coexpressed in a portion of the proximal tubules. These results suggest that hOAT1, hOAT3, and hOCT2 play predominant roles in the transport of organic ions across the basolateral membrane of human proximal tubules.

UR - http://www.scopus.com/inward/record.url?scp=0036205460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036205460&partnerID=8YFLogxK

M3 - Article

C2 - 11912245

AN - SCOPUS:0036205460

VL - 13

SP - 866

EP - 874

JO - Journal of the American Society of Nephrology : JASN

JF - Journal of the American Society of Nephrology : JASN

SN - 1046-6673

IS - 4

ER -