Gene expression of MMP8 and MMP13 during embryonic development of bone and cartilage in the rat mandible and hind limb

Yasuyuki Sasano, Jing Xu Zhu, Makoto Tsubota, Ichiro Takahashi, Kazuyuki Onodera, Itaru Mizoguchi, Manabu Kagayama

Research output: Contribution to journalArticle

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Abstract

Matrix metalloproteinases (MMPs) 8 and 13 comprise the collagenase subfamily in rats and mice, and only MMP13 has been implicated in degradation of the collagenous matrices during development of bone and cartilage. On the hypothesis that MMP8 is also involved in bone and cartilage development, the present study was designed to investigate gene expression of MMP8 in rat embryonic mandibles and hind limbs. Expression of MMP8 was examined with in situ hybridization and RT-PCR and was compared with that of MMP13. Osteoblastic and chondrocytic cells expressing collagenous matrix molecules were identified using in situ hybridization for collagen Types I and II. The results demonstrated that MMP8 is expressed by osteoblastic progenitors, differentiated osteoblasts, osteocytes, and chondrocytes in the growth plate for the first time. Furthermore, the expression of MMP8 is much broader than that of MMP13, for which expression is confined to differentiated phenotypes of osteoblastic and chondrocytic lineage.

Original languageEnglish
Pages (from-to)325-332
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume50
Issue number3
DOIs
Publication statusPublished - Jan 1 2002

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Bone Development
Mandible
Cartilage
Embryonic Development
In Situ Hybridization
Extremities
Matrix Metalloproteinase 8
Matrix Metalloproteinase 13
Gene Expression
Bone and Bones
Osteocytes
Growth Plate
Collagen Type II
Collagenases
Chondrocytes
Collagen Type I
Osteoblasts
Phenotype
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Gene expression of MMP8 and MMP13 during embryonic development of bone and cartilage in the rat mandible and hind limb. / Sasano, Yasuyuki; Zhu, Jing Xu; Tsubota, Makoto; Takahashi, Ichiro; Onodera, Kazuyuki; Mizoguchi, Itaru; Kagayama, Manabu.

In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 3, 01.01.2002, p. 325-332.

Research output: Contribution to journalArticle

Sasano, Yasuyuki ; Zhu, Jing Xu ; Tsubota, Makoto ; Takahashi, Ichiro ; Onodera, Kazuyuki ; Mizoguchi, Itaru ; Kagayama, Manabu. / Gene expression of MMP8 and MMP13 during embryonic development of bone and cartilage in the rat mandible and hind limb. In: Journal of Histochemistry and Cytochemistry. 2002 ; Vol. 50, No. 3. pp. 325-332.
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AU - Onodera, Kazuyuki

AU - Mizoguchi, Itaru

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