Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy

Shigeo Yoshida, Atsushi Ogura, Keijiro Ishikawa, Ayako Yoshida, Richiro Kohno, Yoko Yamaji, Kazuho Ikeo, Takashi Gojobori, Toshihiro Kono, Tatsuro Ishibashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background/aims: The purpose of this study was to generate a profile of genes expressed in preretinal fibrovascular membranes (FVMs) from patients with proliferative diabetic retinopathy. Methods: A PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from FVMs obtained during vitrectomy. The sequence from the 59 end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). Functional annotation was retrieved from Ensemble database and analysed by FatiGO. The web-based VisANT software was used to identify the molecular networks within the FVMs. Results: A total of 2816 ESTs were assembled in 625 non-redundant clusters. Among these, 515 matched the human cDNA database. The 515 clusters were subdivided by functional subsets of genes related to ribosomal activity, oxidative phosphorylation, focal adhesion, cell adhesion and other functions. Querying against the VisANT database yielded 3175 possible physical relationships to other genes/proteins, which included an additional 2480 genes that were not detected in the FVM library. Conclusions: The cDNA library constructed from human FVMs will be a valuable source of information. It should facilitate a wide range of studies that can establish the molecular mechanisms underlying the development of FVMs.

Original languageEnglish
Pages (from-to)795-801
Number of pages7
JournalBritish Journal of Ophthalmology
Volume94
Issue number6
DOIs
Publication statusPublished - Jun 1 2010

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Diabetic Retinopathy
Transcriptome
Membranes
Complementary DNA
Expressed Sequence Tags
Gene Library
Databases
Genes
Focal Adhesions
Oxidative Phosphorylation
Vitrectomy
Nucleic Acid Databases
Cell Adhesion
Libraries
Software
Clone Cells
RNA
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy. / Yoshida, Shigeo; Ogura, Atsushi; Ishikawa, Keijiro; Yoshida, Ayako; Kohno, Richiro; Yamaji, Yoko; Ikeo, Kazuho; Gojobori, Takashi; Kono, Toshihiro; Ishibashi, Tatsuro.

In: British Journal of Ophthalmology, Vol. 94, No. 6, 01.06.2010, p. 795-801.

Research output: Contribution to journalArticle

Yoshida, Shigeo ; Ogura, Atsushi ; Ishikawa, Keijiro ; Yoshida, Ayako ; Kohno, Richiro ; Yamaji, Yoko ; Ikeo, Kazuho ; Gojobori, Takashi ; Kono, Toshihiro ; Ishibashi, Tatsuro. / Gene expression profile of fibrovascular membranes from patients with proliferative diabetic retinopathy. In: British Journal of Ophthalmology. 2010 ; Vol. 94, No. 6. pp. 795-801.
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