Abstract
The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL -1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL -1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL -1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL -1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
| Original language | English |
|---|---|
| Pages (from-to) | 668-674 |
| Number of pages | 7 |
| Journal | Chemosphere |
| Volume | 87 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - May 1 2012 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Environmental Chemistry
- Chemistry(all)
- Pollution
- Health, Toxicology and Mutagenesis
Cite this
Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol. / Hirakawa, Ikumi; Miyagawa, Shinichi; Katsu, Yoshinao; Kagami, Yoshihiro; Tatarazako, Norihisa; Kobayashi, Tohru; Kusano, Teruhiko; Mizutani, Takeshi; Ogino, Yukiko; Takeuchi, Takashi; Ohta, Yasuhiko; Iguchi, Taisen.
In: Chemosphere, Vol. 87, No. 7, 01.05.2012, p. 668-674.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol
AU - Hirakawa, Ikumi
AU - Miyagawa, Shinichi
AU - Katsu, Yoshinao
AU - Kagami, Yoshihiro
AU - Tatarazako, Norihisa
AU - Kobayashi, Tohru
AU - Kusano, Teruhiko
AU - Mizutani, Takeshi
AU - Ogino, Yukiko
AU - Takeuchi, Takashi
AU - Ohta, Yasuhiko
AU - Iguchi, Taisen
PY - 2012/5/1
Y1 - 2012/5/1
N2 - The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL -1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL -1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL -1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL -1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
AB - The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL -1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL -1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL -1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL -1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
UR - http://www.scopus.com/inward/record.url?scp=84858621292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84858621292&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2011.12.047
DO - 10.1016/j.chemosphere.2011.12.047
M3 - Article
C2 - 22230730
AN - SCOPUS:84858621292
VL - 87
SP - 668
EP - 674
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 7
ER -