TY - JOUR
T1 - Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol
AU - Hirakawa, Ikumi
AU - Miyagawa, Shinichi
AU - Katsu, Yoshinao
AU - Kagami, Yoshihiro
AU - Tatarazako, Norihisa
AU - Kobayashi, Tohru
AU - Kusano, Teruhiko
AU - Mizutani, Takeshi
AU - Ogino, Yukiko
AU - Takeuchi, Takashi
AU - Ohta, Yasuhiko
AU - Iguchi, Taisen
N1 - Funding Information:
We are grateful to Dr. Anke Lange, University of Exeter, for her critical readings of the manuscript. This work was supported by UK-Japan Research Collaboration Grants from the Ministry of the Environment, Japan, and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to S.M., Y.K. N.T., T.K. Y.O. and T.I.
PY - 2012/5
Y1 - 2012/5
N2 - The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL-1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL-1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL-1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL-1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
AB - The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL-1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL-1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL-1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL-1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
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U2 - 10.1016/j.chemosphere.2011.12.047
DO - 10.1016/j.chemosphere.2011.12.047
M3 - Article
C2 - 22230730
AN - SCOPUS:84858621292
VL - 87
SP - 668
EP - 674
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 7
ER -