Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol

Ikumi Hirakawa, Shinichi Miyagawa, Yoshinao Katsu, Yoshihiro Kagami, Norihisa Tatarazako, Tohru Kobayashi, Teruhiko Kusano, Takeshi Mizutani, Yukiko Ogino, Takashi Takeuchi, Yasuhiko Ohta, Taisen Iguchi

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31 Citations (Scopus)

Abstract

The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL-1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL-1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL-1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL-1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.

Original languageEnglish
Pages (from-to)668-674
Number of pages7
JournalChemosphere
Volume87
Issue number7
DOIs
Publication statusPublished - May 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Mizutani, T., Ogino, Y., Takeuchi, T., Ohta, Y., & Iguchi, T. (2012). Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol. Chemosphere, 87(7), 668-674. https://doi.org/10.1016/j.chemosphere.2011.12.047