Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol

Ikumi Hirakawa, Shinichi Miyagawa, Yoshinao Katsu, Yoshihiro Kagami, Norihisa Tatarazako, Tohru Kobayashi, Teruhiko Kusano, Takeshi Mizutani, Yukiko Ogino, Takashi Takeuchi, Yasuhiko Ohta, Taisen Iguchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100ngL -1 of 17α-ethinylestradiol (EE2) for 3-5weeks, and 800ng estradiol benzoate (EB) for 3weeks (experiment I), and a measured concentration of 20ngL -1 EE2 for 1-6weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4weeks of exposure to 100ngL -1 EE2, 3weeks of exposure to 800ngEB, and 6weeks of exposure to 20ngL -1 EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.

Original languageEnglish
Pages (from-to)668-674
Number of pages7
JournalChemosphere
Volume87
Issue number7
DOIs
Publication statusPublished - May 1 2012
Externally publishedYes

Fingerprint

Oryzias
Ethinyl Estradiol
Transcriptome
Gene expression
gene expression
Ovum
Testis
Genes
Microarrays
gene
Estrogens
Liver
Microarray Analysis
Vitellogenins
Fish
brain
Brain
Oocytes
experiment
Experiments

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol. / Hirakawa, Ikumi; Miyagawa, Shinichi; Katsu, Yoshinao; Kagami, Yoshihiro; Tatarazako, Norihisa; Kobayashi, Tohru; Kusano, Teruhiko; Mizutani, Takeshi; Ogino, Yukiko; Takeuchi, Takashi; Ohta, Yasuhiko; Iguchi, Taisen.

In: Chemosphere, Vol. 87, No. 7, 01.05.2012, p. 668-674.

Research output: Contribution to journalArticle

Hirakawa, I, Miyagawa, S, Katsu, Y, Kagami, Y, Tatarazako, N, Kobayashi, T, Kusano, T, Mizutani, T, Ogino, Y, Takeuchi, T, Ohta, Y & Iguchi, T 2012, 'Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol', Chemosphere, vol. 87, no. 7, pp. 668-674. https://doi.org/10.1016/j.chemosphere.2011.12.047
Hirakawa, Ikumi ; Miyagawa, Shinichi ; Katsu, Yoshinao ; Kagami, Yoshihiro ; Tatarazako, Norihisa ; Kobayashi, Tohru ; Kusano, Teruhiko ; Mizutani, Takeshi ; Ogino, Yukiko ; Takeuchi, Takashi ; Ohta, Yasuhiko ; Iguchi, Taisen. / Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol. In: Chemosphere. 2012 ; Vol. 87, No. 7. pp. 668-674.
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