TY - JOUR
T1 - Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity
AU - Morita, Sumiyo
AU - Nakabayashi, Kazuhiko
AU - Kawai, Tomoko
AU - Hayashi, Keiko
AU - Horii, Takuro
AU - Kimura, Mika
AU - Kamei, Yasutomi
AU - Ogawa, Yoshihiro
AU - Hata, Kenichiro
AU - Hatada, Izuho
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (MEXT KAKENHI Grant Number: 15K14432).
Publisher Copyright:
© 2016, Nature Publishing Group. All rights reserved.
PY - 2016/2/12
Y1 - 2016/2/12
N2 - Previously, we found that C57BL/6J (B6) mice are more prone to develop obesity than PWK mice. In addition, we analyzed reciprocal crosses between these mice and found that (PWK x B6) F1 mice, which have B6 fathers, are more likely to develop dietary obesity than (B6 x PWK) F1 mice, which have B6 mothers. These results suggested that diet-induced obesity is paternally transmitted. In this study, we performed transcriptome analysis of adipose tissues of B6, PWK, (PWK x B6) F1, and (B6 x PWK) F1 mice using next-generation sequencing. We found that paternal transmission of diet-induced obesity was correlated with genes involved in adipose tissue inflammation, metal ion transport, and cilia. Furthermore, we analyzed the imprinted genes expressed in white adipose tissue (WAT) and obesity. Expression of paternally expressed imprinted genes (PEGs) was negatively correlated with body weight, whereas expression of maternally expressed imprinted genes (MEGs) was positively correlated. In the obesity-prone B6 mice, expression of PEGs was down-regulated by a high-fat diet, suggesting that abnormally low expression of PEGs contributes to high-fat diet-induced obesity in B6 mice. In addition, using single-nucleotide polymorphisms that differ between B6 and PWK, we identified candidate imprinted genes in WAT.
AB - Previously, we found that C57BL/6J (B6) mice are more prone to develop obesity than PWK mice. In addition, we analyzed reciprocal crosses between these mice and found that (PWK x B6) F1 mice, which have B6 fathers, are more likely to develop dietary obesity than (B6 x PWK) F1 mice, which have B6 mothers. These results suggested that diet-induced obesity is paternally transmitted. In this study, we performed transcriptome analysis of adipose tissues of B6, PWK, (PWK x B6) F1, and (B6 x PWK) F1 mice using next-generation sequencing. We found that paternal transmission of diet-induced obesity was correlated with genes involved in adipose tissue inflammation, metal ion transport, and cilia. Furthermore, we analyzed the imprinted genes expressed in white adipose tissue (WAT) and obesity. Expression of paternally expressed imprinted genes (PEGs) was negatively correlated with body weight, whereas expression of maternally expressed imprinted genes (MEGs) was positively correlated. In the obesity-prone B6 mice, expression of PEGs was down-regulated by a high-fat diet, suggesting that abnormally low expression of PEGs contributes to high-fat diet-induced obesity in B6 mice. In addition, using single-nucleotide polymorphisms that differ between B6 and PWK, we identified candidate imprinted genes in WAT.
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U2 - 10.1038/srep21693
DO - 10.1038/srep21693
M3 - Article
C2 - 26868178
AN - SCOPUS:84957990900
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 21693
ER -
