Gene mutation assay using a MutS protein-modified electrode.

Aishan Han, Taiki Shibata, Tohru Takarada, Mizuo Maeda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A novel electrochemical biosensor for gene mutation detection was developed using a DNA mismatch recognizing protein MutS from E. coli. The MutS protein was immobilized onto an Au electrode by coordination of His-tag at its C-terminus to vacant sites of Ni(II)-nitrilotriacetato complex attached to the surface of electrode. When a target DNA duplex having a mismatch site was captured by the MutS protein on the electrode, the electrostatic repulsion arose between polyanionic DNA duplexes and negatively-charged ferrocyanide/ferricyanide redox couple ions. Consequently, their redox peak currents on a cyclic voltammogram with the Au electrode drastically decreased depending on the concentration of the target DNA according to the redox couple-mediated artificial ion-channel principle. Using this assay, we could detect GT mismatch and deletion mutation in the double-stranded DNA.

Original languageEnglish
Pages (from-to)287-288
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
Publication statusPublished - 2002
Externally publishedYes

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Electrodes
Mutation
DNA
Oxidation-Reduction
Genes
Proteins
Immobilized Proteins
Sequence Deletion
Biosensing Techniques
Static Electricity
Ion Channels
Ions

Cite this

Han, A., Shibata, T., Takarada, T., & Maeda, M. (2002). Gene mutation assay using a MutS protein-modified electrode. Nucleic acids research. Supplement (2001), (2), 287-288.

Gene mutation assay using a MutS protein-modified electrode. / Han, Aishan; Shibata, Taiki; Takarada, Tohru; Maeda, Mizuo.

In: Nucleic acids research. Supplement (2001), No. 2, 2002, p. 287-288.

Research output: Contribution to journalArticle

Han, A, Shibata, T, Takarada, T & Maeda, M 2002, 'Gene mutation assay using a MutS protein-modified electrode.', Nucleic acids research. Supplement (2001), no. 2, pp. 287-288.
Han, Aishan ; Shibata, Taiki ; Takarada, Tohru ; Maeda, Mizuo. / Gene mutation assay using a MutS protein-modified electrode. In: Nucleic acids research. Supplement (2001). 2002 ; No. 2. pp. 287-288.
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