Gene replacement of the p53 gene with the lacz gene in mouse embryonic stem-cells and mice by using two steps of homologous recombination

Yoichi Gondo, Kenji Nakamura, Kazuki Nakao, Toshikuni Sasaoka, Kenichi Ito, Minoru Kimura, Motoya Katsuki

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Two steps of gene targeting were used to replace the p53 gene with the E.coli β-galactosidase (lacZ) gene in mouse embryonic stem (ES) cells. The first targeting vector consisted of neo and herpes simplex virus thymidine kinase (HSV-tk) genes as a neo-tk cassette in the middle of the targeting vector. At the first targeting, the homologous recombinants became G418 resistant and ganciclovir (GANC) sensitive and were selected by G418 alone. At the second targeting, homologous recombination reciprocally exchanged the neo-tk cassette in the ES cell chromosome with the lacZ fragment in the second targeting vector and thus made the ES cells GANC resistant. We obtained two ES cell clones, in which the p53 gene for both had been replaced with a totally non-homologous sequence of the lacZ gene. The germ-line transmission of the manipulated ES cells also demonstrated that the entire procedure had no detrimental effects on ES cells at all.

Original languageEnglish
Pages (from-to)830-837
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number2
DOIs
Publication statusPublished - Jul 29 1994

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this