Gene structure and multiple mRNA species of Drosophila melanogaster aldolase generating three isozymes with different enzymatic properties

Tatsuo Kai, Yasushi Sugimoto, Takahiro Kusakabe, Rong Zhang, Katsumi Koga, Katsuji Hori

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Genomic clones encoding the Drosophila aldolase gene were isolated and the organization of the gene was determined. The protein-coding region spanning nearly 3.5 kb consists of five coding exons (exon 2, 3, 4α4β, and Aγ). The insect exon 2 corresponds to exons 2 to 7 of vertebrate aldolase genes and thus appears to have been formed by the fusion of these 6 exons into a single exon daring evolution. The Drosophila aldolase gene is predicted to generate mRNAs for three isozymes (α-, β-, and γ-types) from the primary transcripts by alternative usage of the final three exons. The reverse transcriptase-PCR assay revealed the occurrence of mRNAs for the three isozymic forms at different developmental stages, and tissue-specific expression was also found to occur in adult flies. In addition to the usual type mRNA species for the α-, β-, and γ-isozymes, two novel forms of mRNAs, αbeta;- and βγ-type mRNAs, were detected tissue-specifically in adult files, although their functions are unpredictable. The αβ-mRNA is an α-type mRNA in which exon 4β remains unspliced, while the βγ-mRNA is a β-type. mRNA with the exon 4γ remaining unspliced. Recombinant enzymes expressed in Escherichia coli were all active and exhibited different enzymatic properties.

Original languageEnglish
Pages (from-to)677-688
Number of pages12
JournalJournal of biochemistry
Volume112
Issue number5
DOIs
Publication statusPublished - Nov 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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