Gene structure of human cytochrome P-450(SCC), cholesterol desmolase

Ken-Ichirou Morohashi, Kazuhiro Sogawa, Tsuneo Omura, Yoshiaki Fujii-kuriyama

Research output: Contribution to journalArticle

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Abstract

Four independent clones containing a part of the P-450(SCC), cholesterol desmolase, gene were isolated from human genomic libraries using bovine P-450(SCC) cDNA as a probe. These clones covered the entire P-450(SCC) gene except for a part of the 1st intron. The gene is at least 20 kb long and is split into 9 exons by 8 introns. The sequence analysis revealed that the nine separated exons code for a primary structure consisting of 521 amino acids which shows 72% homology with that of bovine P-450(SCC). A CATT sequence and a TATAAT sequence, which are possibly a "CAT" box, and a "TATA" box, respectively, are present 129 and 91 bp upstream from the initiation codon. An unusual exon/intron junctional sequence that begins with GC was found in the 6th intron of the gene. A putative extension peptide consisting of 39 amino acids was found in the sequence of human P-450(SCC) by comparison with that of the bovine counterpart. Two conserved regions were found in the extension peptide of these two forms of P-450(SCC), suggesting a functional role of the portions in the mitochondrial localization and processing of P-450(SCC) precursor. The mature form of human P-450(SCC) has only one cysteine residue, which was located in the center of the HR2 region (Gotoh et al. (1983) J. Biochem. 97, 807-817). This observation established beyond doubt that the sole cysteine residue in the HR2 region is the 5th ligand to the heme.

Original languageEnglish
Pages (from-to)879-887
Number of pages9
JournalJournal of biochemistry
Volume101
Issue number4
DOIs
Publication statusPublished - Jan 1 1987

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Cholesterol Side-Chain Cleavage Enzyme
Introns
Cytochrome P-450 Enzyme System
Genes
Exons
Cysteine
Clone Cells
Amino Acids
Peptides
TATA Box
Initiator Codon
Genomic Library
Heme
Sequence Analysis
Complementary DNA
Ligands
Processing

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Gene structure of human cytochrome P-450(SCC), cholesterol desmolase. / Morohashi, Ken-Ichirou; Sogawa, Kazuhiro; Omura, Tsuneo; Fujii-kuriyama, Yoshiaki.

In: Journal of biochemistry, Vol. 101, No. 4, 01.01.1987, p. 879-887.

Research output: Contribution to journalArticle

Morohashi, Ken-Ichirou ; Sogawa, Kazuhiro ; Omura, Tsuneo ; Fujii-kuriyama, Yoshiaki. / Gene structure of human cytochrome P-450(SCC), cholesterol desmolase. In: Journal of biochemistry. 1987 ; Vol. 101, No. 4. pp. 879-887.
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