TY - JOUR
T1 - Gene transfection into HeLa cells by vesicles containing cationic peptide lipid
AU - Ohama, Yumi
AU - Heiki, Yuji
AU - Sugahara, Takuya
AU - Sakata, Kozue
AU - Yoshimura, Norikazu
AU - Hisaeda, Yoshio
AU - Hosokawa, Mami
AU - Takashima, Shigemitsu
AU - Kato, Keiichi
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research from the Ministry of Health, Labor, and Welfare of Japan, and by a Grant-in-Aid (to K.K.) (nos. 08650914 and 10450297) from Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2005/8/23
Y1 - 2005/8/23
N2 - Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.
AB - Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.
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U2 - 10.1271/bbb.69.1453
DO - 10.1271/bbb.69.1453
M3 - Article
C2 - 16116271
AN - SCOPUS:23944508993
VL - 69
SP - 1453
EP - 1458
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 8
ER -