Gene transfection into HeLa cells by vesicles containing cationic peptide lipid

Yumi Ohama, Yuji Heiki, Takuya Sugahara, Kozue Sakata, Norikazu Yoshimura, Yoshio Hisaeda, Mami Hosokawa, Shigemitsu Takashima, Keiichi Kato

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.

    Original languageEnglish
    Pages (from-to)1453-1458
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume69
    Issue number8
    DOIs
    Publication statusPublished - Aug 23 2005

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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