Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy

Naoki Miyano, Yuuki Inoue, Yuji Teramura, Keisuke Fujii, Fujio Tsumori, Hiroo Iwata, Hidetoshi Kotera

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the diffusional phase transformation of two-phase alloys, the new phase precipitates form the matrix phase at specific temperatures, followed by the formation of a mixed microstructure comprising the precipitate and the matrix. It has been found that by specific chemical-etching treatment, the precipitate in Fe-25Cr-6Ni alloy projects substantially and clusters at the surface. The configuration of the precipitate has an extremely high aspect ratio: it is several microns in width and several tens of microns in length (known as micron-spiked). This study targets the development of a gene transfer device with a micro-spike produced based on the self-organization phenomenon of the Fe-25Cr-6Ni alloy. With this spike-projected device, we tried to efficiently transfer plasmid DNA into adherent cells by electric pulse-triggered gene transfer using a plasmid-loaded electrode (electroporation-based reverse transfection). The spiked structure was applied to a substrate of the device to allow efficient gene transfer into adherent cells, although the general substrate was flat and had a smooth surface. The results suggest that this unique spike-projected device has potential applications in gene transfer devices for the analysis of the human genome in the post-genome period.

Original languageEnglish
Pages (from-to)1104-1109
Number of pages6
JournalLab on a Chip
Volume8
Issue number7
DOIs
Publication statusPublished - Jan 1 2008

Fingerprint

Gene transfer
Precipitates
Electrodes
Equipment and Supplies
Genes
Plasmids
Substrates
Electroporation
Aspect ratio
Human Genome
Etching
DNA
Phase transitions
Transfection
Microstructure
Genome
Temperature

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy. / Miyano, Naoki; Inoue, Yuuki; Teramura, Yuji; Fujii, Keisuke; Tsumori, Fujio; Iwata, Hiroo; Kotera, Hidetoshi.

In: Lab on a Chip, Vol. 8, No. 7, 01.01.2008, p. 1104-1109.

Research output: Contribution to journalArticle

Miyano, Naoki ; Inoue, Yuuki ; Teramura, Yuji ; Fujii, Keisuke ; Tsumori, Fujio ; Iwata, Hiroo ; Kotera, Hidetoshi. / Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy. In: Lab on a Chip. 2008 ; Vol. 8, No. 7. pp. 1104-1109.
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