Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

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Abstract

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

Original languageEnglish
Pages (from-to)1227-1229
Number of pages3
JournalOptics Letters
Volume29
Issue number11
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Terakawa, M., Ogura, M., Sato, S., Wakisaka, H., Ashida, H., Uenoyama, M., ... Obara, M. (2004). Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave. Optics Letters, 29(11), 1227-1229. https://doi.org/10.1364/OL.29.001227