Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa; Makoto Ogura; Shunichi Sato; Hitoshi Wakisaka; Hiroshi Ashida; Maki Uenoyama; Yoshinori Masaki; Minoru Obara

doi:10.1364/OL.29.001227

Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

Original language	English
Pages (from-to)	1227-1229
Number of pages	3
Journal	Optics Letters
Volume	29
Issue number	11
DOIs	https://doi.org/10.1364/OL.29.001227
Publication status	Published - Jun 1 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OL.29.001227

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abstract = "Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.",

author = "Mitsuhiro Terakawa and Makoto Ogura and Shunichi Sato and Hitoshi Wakisaka and Hiroshi Ashida and Maki Uenoyama and Yoshinori Masaki and Minoru Obara",

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AU - Terakawa, Mitsuhiro

AU - Ogura, Makoto

AU - Sato, Shunichi

AU - Wakisaka, Hitoshi

AU - Ashida, Hiroshi

AU - Uenoyama, Maki

AU - Masaki, Yoshinori

AU - Obara, Minoru

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

AB - Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

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Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Abstract

All Science Journal Classification (ASJC) codes

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