Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa; Makoto Ogura; Shunichi Sato; Hitoshi Wakisaka; Hiroshi Ashida; Maki Uenoyama; Yoshinori Masaki; Minoru Obara

doi:10.1364/OL.29.001227

Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

Research output: Contribution to journal › Article

58 Citations (Scopus)

Abstract

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

Original language	English
Pages (from-to)	1227-1229
Number of pages	3
Journal	Optics Letters
Volume	29
Issue number	11
DOIs	https://doi.org/10.1364/OL.29.001227
Publication status	Published - Jun 1 2004
Externally published	Yes

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All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

Cite this

Terakawa, M., Ogura, M., Sato, S., Wakisaka, H., Ashida, H., Uenoyama, M., ... Obara, M. (2004). Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave. Optics Letters, 29(11), 1227-1229. https://doi.org/10.1364/OL.29.001227

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10.1364/OL.29.001227