Abstract
Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 421-424 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 231 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 13 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate. / Emi, Nobuhiko; Kidoaki, Satoru; Yoshikawa, Kenichi; Saito, Hidehiko.
In: Biochemical and Biophysical Research Communications, Vol. 231, No. 2, 13.02.1997, p. 421-424.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate
AU - Emi, Nobuhiko
AU - Kidoaki, Satoru
AU - Yoshikawa, Kenichi
AU - Saito, Hidehiko
PY - 1997/2/13
Y1 - 1997/2/13
N2 - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
AB - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031566270&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.6125
DO - 10.1006/bbrc.1997.6125
M3 - Article
VL - 231
SP - 421
EP - 424
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -