Abstract
Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 421-424 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 231 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 13 1997 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Emi, N., Kidoaki, S., Yoshikawa, K., & Saito, H. (1997). Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate. Biochemical and Biophysical Research Communications, 231(2), 421-424. https://doi.org/10.1006/bbrc.1997.6125