Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate

Nobuhiko Emi; Satoru Kidoaki; Kenichi Yoshikawa; Hidehiko Saito

doi:10.1006/bbrc.1997.6125

Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate

Nobuhiko Emi, Satoru Kidoaki, Kenichi Yoshikawa, Hidehiko Saito

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.

Original language	English
Pages (from-to)	421-424
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	231
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1997.6125
Publication status	Published - Feb 13 1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1997.6125

Fingerprint Dive into the research topics of 'Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate'. Together they form a unique fingerprint.

Cite this

@article{1d008bc1b9e44136b4fc0a71bfb80a4e,

title = "Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate",

abstract = "Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.",

author = "Nobuhiko Emi and Satoru Kidoaki and Kenichi Yoshikawa and Hidehiko Saito",

year = "1997",

month = feb,

day = "13",

doi = "10.1006/bbrc.1997.6125",

language = "English",

volume = "231",

pages = "421--424",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate

AU - Emi, Nobuhiko

AU - Kidoaki, Satoru

AU - Yoshikawa, Kenichi

AU - Saito, Hidehiko

PY - 1997/2/13

Y1 - 1997/2/13

N2 - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.

AB - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.

UR - http://www.scopus.com/inward/record.url?scp=0031566270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031566270&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.6125

DO - 10.1006/bbrc.1997.6125

M3 - Article

C2 - 9070292

AN - SCOPUS:0031566270

VL - 231

SP - 421

EP - 424

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Cite this