Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate

Nobuhiko Emi, Satoru Kidoaki, Kenichi Yoshikawa, Hidehiko Saito

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number2
DOIs
Publication statusPublished - Feb 13 1997
Externally publishedYes

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Gene transfer
Transfection
Arginine
DNA
Genes
Fluorescence microscopy
Fluorescence
Fluorescence Microscopy
Peptides
Light
polyarginine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate. / Emi, Nobuhiko; Kidoaki, Satoru; Yoshikawa, Kenichi; Saito, Hidehiko.

In: Biochemical and Biophysical Research Communications, Vol. 231, No. 2, 13.02.1997, p. 421-424.

Research output: Contribution to journalArticle

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