TY - JOUR
T1 - Gene transfer mediated by polyarginine requires a formation of big carrier-complex of DNA aggregate
AU - Emi, Nobuhiko
AU - Kidoaki, Satoru
AU - Yoshikawa, Kenichi
AU - Saito, Hidehiko
N1 - Funding Information:
The present study was supported in part by a Grant-in-Aid for Scienti®c Research from the Ministry of Education, Science and Culture of Japan, and from the Science and Technology Agency of the Japanese Government.
PY - 1997/2/13
Y1 - 1997/2/13
N2 - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
AB - Cationic peptide, poly-arginine, promotes transfection efficiency under a proper concentration. Using fluorescence microscopy, we observed the structure of the DNA carrier-complex in solution directly. Under low concentration of poly-arginine, small complexes were observed with fluorescence images like a blight light spot. This small complex (sizes are c.a. 1~2 μm order) had no transfection ability. As the concentration of poly-arginine became high, the complex grew into a big one whose size reached to a degree of 10 μm. Transfection was succeeded under that condition. The relationship between transfection efficiency and higher-order structure of the DNA carrier-complex was discussed.
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U2 - 10.1006/bbrc.1997.6125
DO - 10.1006/bbrc.1997.6125
M3 - Article
C2 - 9070292
AN - SCOPUS:0031566270
VL - 231
SP - 421
EP - 424
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -