Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100

Takuya Imamura, Naka Hattori, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.

Original languageEnglish
Pages (from-to)534-539
Number of pages6
JournalJournal of Biochemistry
Volume124
Issue number3
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

Fingerprint

Sialoglycoproteins
Brain
Monoclonal Antibodies
Antigens
Molecules
Antibodies
Glycoproteins
Glycoside Hydrolases
Membrane Glycoproteins
Neurogenesis
Isoelectric Point
Molecular mass
Prosencephalon
Immunoblotting
Lectins
Rats
Parturition
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100. / Imamura, Takuya; Hattori, Naka; Shiota, Kunio; Ogawa, Tomoya.

In: Journal of Biochemistry, Vol. 124, No. 3, 01.01.1998, p. 534-539.

Research output: Contribution to journalArticle

@article{dfda39c94e414423ac03dab7149061b8,
title = "Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100",
abstract = "With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.",
author = "Takuya Imamura and Naka Hattori and Kunio Shiota and Tomoya Ogawa",
year = "1998",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.jbchem.a022145",
language = "English",
volume = "124",
pages = "534--539",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100

AU - Imamura, Takuya

AU - Hattori, Naka

AU - Shiota, Kunio

AU - Ogawa, Tomoya

PY - 1998/1/1

Y1 - 1998/1/1

N2 - With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.

AB - With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.

UR - http://www.scopus.com/inward/record.url?scp=0031690576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031690576&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a022145

DO - 10.1093/oxfordjournals.jbchem.a022145

M3 - Article

C2 - 9722662

AN - SCOPUS:0031690576

VL - 124

SP - 534

EP - 539

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 3

ER -