Generation and characterization of T1R2-LacZ knock-in mouse

Ken Iwatsuki, Masatoshi Nomura, Atsushi Shibata, Reiko Ichikawa, Patricio L.M. Enciso, Lixiang Wang, Ryoichi Takayanagi, Kunio Torii, Hisayuki Uneyama

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38 Citations (Scopus)

Abstract

Taste cells are chemosensory epithelial cells that sense distinct taste quality such as umami, sweet, bitter, sour and salty. Taste cells utilize G protein-coupled receptors to detect umami, sweet and bitter taste whereas ion channels are responsible for detecting salty and sour taste. Among these taste receptors, taste receptor type 2, T1R2 (or Tas1r2), has been identified as a sole sweet taste receptor in mammals that mediates sweet signals upon dimerization with T1R3. However, because of limited availability of reliable antibodies and low expression level of G protein-coupled receptors, it is uneasy to identify the cell-types that express these receptors in non-taste tissues. In this study, we have generated a T1R2-LacZ reporter knock-in mouse to investigate tissue distribution of T1R2 at a single-cell level. We found that the LacZ gene expression in these mice was faithful to the expression of T1R2 in the taste tissue and in the gastrointestinal tract where T1R3 expression has been reported. Surprisingly, T1R2 expression was also found in the testis. Mice homozygous for T1R2 deletion lacked T1R2 protein analyzed by the antibody raised against T1R2 peptide sequences. In summary, the T1R2 knock-in mouse is a powerful tool to analyze the putative targets for sweeteners as well as to study the physiological roles of T1R2 in detecting sugars.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume402
Issue number3
DOIs
Publication statusPublished - Nov 19 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Iwatsuki, K., Nomura, M., Shibata, A., Ichikawa, R., Enciso, P. L. M., Wang, L., Takayanagi, R., Torii, K., & Uneyama, H. (2010). Generation and characterization of T1R2-LacZ knock-in mouse. Biochemical and Biophysical Research Communications, 402(3), 495-499. https://doi.org/10.1016/j.bbrc.2010.10.057