Generation and development of RNA ligase ribozymes with modular architecture through "design and selection"

Yuki Fujita, Junya Ishikawa, Hiroyuki Furuta, Yoshiya Ikawa

    Research output: Contribution to journalReview articlepeer-review

    8 Citations (Scopus)

    Abstract

    In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules. Rational RNA design is an attractive alternative to avoid this laborious step, but rational design of catalytic modules is still a challenging task. A hybrid strategy of in vitro selection and rational design has been proposed. With this strategy termed "design and selection," new ribozymes can be generated through installation of catalytic modules onto RNA scaffolds with defined 3D structures. This approach, the concept of which was inspired by the modular architecture of naturally occurring ribozymes, allows prediction of the overall architectures of the resulting ribozymes, and the structural modularity of the resulting ribozymes allows modification of their structures and functions. In this review, we summarize the design, generation, properties, and engineering of four classes of ligase ribozyme generated by design and selection.

    Original languageEnglish
    Pages (from-to)5850-5865
    Number of pages16
    JournalMolecules
    Volume15
    Issue number9
    DOIs
    Publication statusPublished - Sep 2010

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Chemistry (miscellaneous)
    • Molecular Medicine
    • Pharmaceutical Science
    • Drug Discovery
    • Physical and Theoretical Chemistry
    • Organic Chemistry

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