Generation of a human anti-tumor necrosis factor-α monoclonal antibody by in vitro immunization with a multiple antigen peptide

Tsukasa Fujiki, Atsuji Tsun, Shin Ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Sanetaka Shirahata, Yoshinori Katakura

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We developed the in vitro immunization method to induce antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs). However, when we used a peptide as sensitizing antigen, the antigen-specific immune response was found to be weak, and hence, we could not effectively obtain the antigen-specific antibody gene. In the present study, we attempted to improve the in vitro immunization method by augmenting the immune response to the peptide antigen. We used a multiple antigen peptide for sensitization. In vitro immunization of the multivalent antigen elicited a strong antigen-specific immune response in the PBMCs, and we succeeded in obtaining antigen-specific antibody genes by the phage-display method. Further, by combining the variable-region genes and constant-region genes of human IgG, we obtained four independent human monoclonal antibodies specific for tumor necrosis factor-a. This might be a good strategy for generating antigen-specific human monoclonal antibodies using a peptide antigen.

Original languageEnglish
Pages (from-to)1836-1840
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number9
Publication statusPublished - Oct 8 2010


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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