Generation of a human anti-tumor necrosis factor-α monoclonal antibody by in vitro immunization with a multiple antigen peptide

Tsukasa Fujiki, Atsuji Tsun, Shin Ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Sanetaka Shirahata, Yoshinori Katakura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We developed the in vitro immunization method to induce antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs). However, when we used a peptide as sensitizing antigen, the antigen-specific immune response was found to be weak, and hence, we could not effectively obtain the antigen-specific antibody gene. In the present study, we attempted to improve the in vitro immunization method by augmenting the immune response to the peptide antigen. We used a multiple antigen peptide for sensitization. In vitro immunization of the multivalent antigen elicited a strong antigen-specific immune response in the PBMCs, and we succeeded in obtaining antigen-specific antibody genes by the phage-display method. Further, by combining the variable-region genes and constant-region genes of human IgG, we obtained four independent human monoclonal antibodies specific for tumor necrosis factor-a. This might be a good strategy for generating antigen-specific human monoclonal antibodies using a peptide antigen.

Original languageEnglish
Pages (from-to)1836-1840
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number9
DOIs
Publication statusPublished - Oct 8 2010

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Immunization
Tumor Necrosis Factor-alpha
Monoclonal Antibodies
Antigens
Peptides
Histocompatibility Antigens Class II
Genes
Blood Cells
human TNF protein
In Vitro Techniques
Blood
Antibodies
Bacteriophages
Immunoglobulin G
Display devices

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Generation of a human anti-tumor necrosis factor-α monoclonal antibody by in vitro immunization with a multiple antigen peptide. / Fujiki, Tsukasa; Tsun, Atsuji; Matsumoto, Shin Ei; Yamashita, Makiko; Teruya, Kiichiro; Shirahata, Sanetaka; Katakura, Yoshinori.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 9, 08.10.2010, p. 1836-1840.

Research output: Contribution to journalArticle

Fujiki, Tsukasa ; Tsun, Atsuji ; Matsumoto, Shin Ei ; Yamashita, Makiko ; Teruya, Kiichiro ; Shirahata, Sanetaka ; Katakura, Yoshinori. / Generation of a human anti-tumor necrosis factor-α monoclonal antibody by in vitro immunization with a multiple antigen peptide. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 9. pp. 1836-1840.
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