Generation of a monoclonal antibody for INI1/hSNF5/BAF47

Akihito Harada, Masayasu Hayashi, Yuuki Kuniyoshi, Yuichiro Semba, Satoko Sugahara, Taro Tachibana, Yasuyuki Ohkawa, Masatoshi Fujita

Research output: Contribution to journalArticle

Abstract

INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.

Original languageEnglish
Pages (from-to)49-51
Number of pages3
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume33
Issue number1
DOIs
Publication statusPublished - Feb 1 2014

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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