Generation of a monoclonal antibody for INI1/hSNF5/BAF47

Akihito Harada, Masayasu Hayashi, Yuuki Kuniyoshi, Yuichiro Semba, Satoko Sugahara, Taro Tachibana, Yasuyuki Ohkawa, Masatoshi Fujita

Research output: Contribution to journalArticle

Abstract

INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.

Original languageEnglish
Pages (from-to)49-51
Number of pages3
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume33
Issue number1
DOIs
Publication statusPublished - Feb 1 2014

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Cell Differentiation
Monoclonal Antibodies
Chromatin Assembly and Disassembly
Antibodies
Gene Expression Regulation
Regulator Genes
Immunoblotting
Protein Isoforms
Adenosine Triphosphate
Lymph Nodes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Generation of a monoclonal antibody for INI1/hSNF5/BAF47. / Harada, Akihito; Hayashi, Masayasu; Kuniyoshi, Yuuki; Semba, Yuichiro; Sugahara, Satoko; Tachibana, Taro; Ohkawa, Yasuyuki; Fujita, Masatoshi.

In: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, Vol. 33, No. 1, 01.02.2014, p. 49-51.

Research output: Contribution to journalArticle

Harada, Akihito ; Hayashi, Masayasu ; Kuniyoshi, Yuuki ; Semba, Yuichiro ; Sugahara, Satoko ; Tachibana, Taro ; Ohkawa, Yasuyuki ; Fujita, Masatoshi. / Generation of a monoclonal antibody for INI1/hSNF5/BAF47. In: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2014 ; Vol. 33, No. 1. pp. 49-51.
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AU - Tachibana, Taro

AU - Ohkawa, Yasuyuki

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