Abstract
We reported the existence of high and low responders to the streptococcal cell wall antigen (SCW) in the human population. To analyze the mechanism of the low responsiveness to SCW at the cellular level, we established SCW‐specific CD4+ T cell lines. During the course of generation of a SCW‐specific CD4+ T cell line restricted by HLA‐DQ from a low responder, we obtained autoreactive CD8+ cytotoxic T lymphocytes as a cell line (HYCD8). They proliferated in the presence of autologous monocytes and IL‐2, without SCW. HYCD8 lysed autologous monocytes and Epstein‐Barr virus‐transformed B lymphoblastoid cell line (BLCL). This cytotoxic activity was specifically inhibited by an anti‐HLA class I framework monoclonal antibody and restricted by HLA‐B52 or B54 specificity, as judged by killing activity against panel cells and HLA class I‐transfected BLCL. It was unique to HYCD8 that the HLA class I‐restricted cytotoxicity was observed only in the presence of soluble factor with low molecular mass (< 104 Da) produced mainly by B cells, which could not be replaced by known cytokines and their mixtures. We thus describe novel HLA class I‐restricted cytotoxic CD8+ T cells that kill antigen‐presenting cells in a soluble factor‐dependent manner.
| Original language | English |
|---|---|
| Pages (from-to) | 3173-3180 |
| Number of pages | 8 |
| Journal | European Journal of Immunology |
| Volume | 23 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
Generation of a novel CD8+ cytotoxic T lymphocyte that requires soluble factor to lyse autologous antigen‐presenting cells. / Yoshizumi, Hideyuki; Kamikawaji, Nobuhiro; Nishimura, Yasuharu; Sasazuki, Takehiko.
In: European Journal of Immunology, Vol. 23, No. 12, 01.01.1993, p. 3173-3180.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Generation of a novel CD8+ cytotoxic T lymphocyte that requires soluble factor to lyse autologous antigen‐presenting cells
AU - Yoshizumi, Hideyuki
AU - Kamikawaji, Nobuhiro
AU - Nishimura, Yasuharu
AU - Sasazuki, Takehiko
PY - 1993/1/1
Y1 - 1993/1/1
N2 - We reported the existence of high and low responders to the streptococcal cell wall antigen (SCW) in the human population. To analyze the mechanism of the low responsiveness to SCW at the cellular level, we established SCW‐specific CD4+ T cell lines. During the course of generation of a SCW‐specific CD4+ T cell line restricted by HLA‐DQ from a low responder, we obtained autoreactive CD8+ cytotoxic T lymphocytes as a cell line (HYCD8). They proliferated in the presence of autologous monocytes and IL‐2, without SCW. HYCD8 lysed autologous monocytes and Epstein‐Barr virus‐transformed B lymphoblastoid cell line (BLCL). This cytotoxic activity was specifically inhibited by an anti‐HLA class I framework monoclonal antibody and restricted by HLA‐B52 or B54 specificity, as judged by killing activity against panel cells and HLA class I‐transfected BLCL. It was unique to HYCD8 that the HLA class I‐restricted cytotoxicity was observed only in the presence of soluble factor with low molecular mass (< 104 Da) produced mainly by B cells, which could not be replaced by known cytokines and their mixtures. We thus describe novel HLA class I‐restricted cytotoxic CD8+ T cells that kill antigen‐presenting cells in a soluble factor‐dependent manner.
AB - We reported the existence of high and low responders to the streptococcal cell wall antigen (SCW) in the human population. To analyze the mechanism of the low responsiveness to SCW at the cellular level, we established SCW‐specific CD4+ T cell lines. During the course of generation of a SCW‐specific CD4+ T cell line restricted by HLA‐DQ from a low responder, we obtained autoreactive CD8+ cytotoxic T lymphocytes as a cell line (HYCD8). They proliferated in the presence of autologous monocytes and IL‐2, without SCW. HYCD8 lysed autologous monocytes and Epstein‐Barr virus‐transformed B lymphoblastoid cell line (BLCL). This cytotoxic activity was specifically inhibited by an anti‐HLA class I framework monoclonal antibody and restricted by HLA‐B52 or B54 specificity, as judged by killing activity against panel cells and HLA class I‐transfected BLCL. It was unique to HYCD8 that the HLA class I‐restricted cytotoxicity was observed only in the presence of soluble factor with low molecular mass (< 104 Da) produced mainly by B cells, which could not be replaced by known cytokines and their mixtures. We thus describe novel HLA class I‐restricted cytotoxic CD8+ T cells that kill antigen‐presenting cells in a soluble factor‐dependent manner.
UR - http://www.scopus.com/inward/record.url?scp=0027482089&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027482089&partnerID=8YFLogxK
U2 - 10.1002/eji.1830231220
DO - 10.1002/eji.1830231220
M3 - Article
C2 - 8258332
AN - SCOPUS:0027482089
VL - 23
SP - 3173
EP - 3180
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 12
ER -