Abstract
Brm is a subunit of the SWI/SNF complex that has a ATPase activity. It is well known that the complex plays a major role in cell processes, such as proliferation, differentiation, and DNA repair of cells. Here we report the production of monoclonal antibody (1H7A10) against Brm by rat medial iliac lymph node method. Immunoblot analysis with the antibody revealed the specific recognition of Brm and increase of Brm protein level in skeletal muscle differentiation. Immunocytochemistry analysis shows nuclear localization in myoblast C2C12 and involvement of transcription in the late stages of differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 455-458 |
| Number of pages | 4 |
| Journal | Hybridoma |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2009 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Immunology and Allergy
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Generation of a rat monoclonal antibody specific for Brm. / Okada, Seiji; Harada, Akihito; Saiwai, Hirokazu; Nakamura, Mako; Ohkawa, Yasuyuki.
In: Hybridoma, Vol. 28, No. 6, 01.12.2009, p. 455-458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of a rat monoclonal antibody specific for Brm
AU - Okada, Seiji
AU - Harada, Akihito
AU - Saiwai, Hirokazu
AU - Nakamura, Mako
AU - Ohkawa, Yasuyuki
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Brm is a subunit of the SWI/SNF complex that has a ATPase activity. It is well known that the complex plays a major role in cell processes, such as proliferation, differentiation, and DNA repair of cells. Here we report the production of monoclonal antibody (1H7A10) against Brm by rat medial iliac lymph node method. Immunoblot analysis with the antibody revealed the specific recognition of Brm and increase of Brm protein level in skeletal muscle differentiation. Immunocytochemistry analysis shows nuclear localization in myoblast C2C12 and involvement of transcription in the late stages of differentiation.
AB - Brm is a subunit of the SWI/SNF complex that has a ATPase activity. It is well known that the complex plays a major role in cell processes, such as proliferation, differentiation, and DNA repair of cells. Here we report the production of monoclonal antibody (1H7A10) against Brm by rat medial iliac lymph node method. Immunoblot analysis with the antibody revealed the specific recognition of Brm and increase of Brm protein level in skeletal muscle differentiation. Immunocytochemistry analysis shows nuclear localization in myoblast C2C12 and involvement of transcription in the late stages of differentiation.
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U2 - 10.1089/hyb.2009.0044
DO - 10.1089/hyb.2009.0044
M3 - Article
VL - 28
SP - 455
EP - 458
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 6
ER -