Generation of colonic IgA-secreting cells in the caecal patch

Kazunori Masahata, Eiji Umemoto, Hisako Kayama, Manato Kotani, Shota Nakamura, Takashi Kurakawa, Junichi Kikuta, Kazuyoshi Gotoh, Daisuke Motooka, Shintaro Sato, Tomonori Higuchi, Yoshihiro Baba, Tomohiro Kurosaki, Makoto Kinoshita, Yosuke Shimada, Taishi Kimura, Ryu Okumura, Akira Takeda, Masaru Tajima, Osamu YoshieMasahiro Fukuzawa, Hiroshi Kiyono, Sidonia Fagarasan, Tetsuya Iida, Masaru Ishii, Kiyoshi Takeda

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

Original languageEnglish
Number of pages1
JournalNature communications
Volume5
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Immunoglobulin A
intestines
cells
Large Intestine
Small Intestine
Peyer's Patches
mice
Lymphoid Tissue
Tissue
Appendectomy
Adoptive Transfer
Microbiota
Appendix
Bacteria
Dendritic Cells
Cells
bacteria
B-Lymphocytes
Chemical analysis
Experiments

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Masahata, K., Umemoto, E., Kayama, H., Kotani, M., Nakamura, S., Kurakawa, T., ... Takeda, K. (2014). Generation of colonic IgA-secreting cells in the caecal patch. Nature communications, 5. https://doi.org/10.1038/ncomms4704

Generation of colonic IgA-secreting cells in the caecal patch. / Masahata, Kazunori; Umemoto, Eiji; Kayama, Hisako; Kotani, Manato; Nakamura, Shota; Kurakawa, Takashi; Kikuta, Junichi; Gotoh, Kazuyoshi; Motooka, Daisuke; Sato, Shintaro; Higuchi, Tomonori; Baba, Yoshihiro; Kurosaki, Tomohiro; Kinoshita, Makoto; Shimada, Yosuke; Kimura, Taishi; Okumura, Ryu; Takeda, Akira; Tajima, Masaru; Yoshie, Osamu; Fukuzawa, Masahiro; Kiyono, Hiroshi; Fagarasan, Sidonia; Iida, Tetsuya; Ishii, Masaru; Takeda, Kiyoshi.

In: Nature communications, Vol. 5, 01.01.2014.

Research output: Contribution to journalArticle

Masahata, K, Umemoto, E, Kayama, H, Kotani, M, Nakamura, S, Kurakawa, T, Kikuta, J, Gotoh, K, Motooka, D, Sato, S, Higuchi, T, Baba, Y, Kurosaki, T, Kinoshita, M, Shimada, Y, Kimura, T, Okumura, R, Takeda, A, Tajima, M, Yoshie, O, Fukuzawa, M, Kiyono, H, Fagarasan, S, Iida, T, Ishii, M & Takeda, K 2014, 'Generation of colonic IgA-secreting cells in the caecal patch', Nature communications, vol. 5. https://doi.org/10.1038/ncomms4704
Masahata K, Umemoto E, Kayama H, Kotani M, Nakamura S, Kurakawa T et al. Generation of colonic IgA-secreting cells in the caecal patch. Nature communications. 2014 Jan 1;5. https://doi.org/10.1038/ncomms4704
Masahata, Kazunori ; Umemoto, Eiji ; Kayama, Hisako ; Kotani, Manato ; Nakamura, Shota ; Kurakawa, Takashi ; Kikuta, Junichi ; Gotoh, Kazuyoshi ; Motooka, Daisuke ; Sato, Shintaro ; Higuchi, Tomonori ; Baba, Yoshihiro ; Kurosaki, Tomohiro ; Kinoshita, Makoto ; Shimada, Yosuke ; Kimura, Taishi ; Okumura, Ryu ; Takeda, Akira ; Tajima, Masaru ; Yoshie, Osamu ; Fukuzawa, Masahiro ; Kiyono, Hiroshi ; Fagarasan, Sidonia ; Iida, Tetsuya ; Ishii, Masaru ; Takeda, Kiyoshi. / Generation of colonic IgA-secreting cells in the caecal patch. In: Nature communications. 2014 ; Vol. 5.
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