Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal cells

Sahoko Matsuoka, Kohichiro Tsuji, Hiroaki Hisakawa, Ming Jiang Xu, Yasuhiro Ebihara, Takefumi Ishii, Daisuke Sugiyama, Atsushi Manabe, Ryuhei Tanaka, Yasuo Ikeda, Shigetaka Asano, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

There is controversy as to whether murine definitive hematopoiesis originates from yolk sac (YS) or the intraembryonic region. This study reports the generation of definitive hematopoietic stem cells (HSCs) from both early YS and intraembryonic paraaortic splanchnopleures (P-Sp) on AGM-S3 stromal cells derived from the aorta-gonad-mesonephros (AGM) region at 10.5 days post coitum (dpc). YS and P-Sp cells at 8.5 dpc generated no definitive hematopoiesis-derived colony-forming cells in cocultures with AGM-S3 cells, but spleen colony-forming cells and HSCs capable of reconstituting definitive hematopoiesis in adult mice simultaneously appeared on day 4 of coculture. Precursors for definitive HSCs were present in YS and P-Sp at 8.0 dpc, a time when YS and embryo were not connected by blood vessels. It is proposed that precursors with the potential to generate definitive HSCs appear independently in YS and intraembryonic P-Sp and that the P-Sp or AGM region affords the microenvironment that facilitates generation of definitive hematopoiesis from precursors.

Original languageEnglish
Pages (from-to)6-12
Number of pages7
JournalBlood
Volume98
Issue number1
DOIs
Publication statusPublished - Jul 1 2001
Externally publishedYes

Fingerprint

Mesonephros
Yolk Sac
Gonads
Stromal Cells
Hematopoietic Stem Cells
Stem cells
Aorta
Hematopoiesis
Coculture Techniques
Blood vessels
Blood Vessels
Spleen
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal cells. / Matsuoka, Sahoko; Tsuji, Kohichiro; Hisakawa, Hiroaki; Xu, Ming Jiang; Ebihara, Yasuhiro; Ishii, Takefumi; Sugiyama, Daisuke; Manabe, Atsushi; Tanaka, Ryuhei; Ikeda, Yasuo; Asano, Shigetaka; Nakahata, Tatsutoshi.

In: Blood, Vol. 98, No. 1, 01.07.2001, p. 6-12.

Research output: Contribution to journalArticle

Matsuoka, S, Tsuji, K, Hisakawa, H, Xu, MJ, Ebihara, Y, Ishii, T, Sugiyama, D, Manabe, A, Tanaka, R, Ikeda, Y, Asano, S & Nakahata, T 2001, 'Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal cells', Blood, vol. 98, no. 1, pp. 6-12. https://doi.org/10.1182/blood.V98.1.6
Matsuoka, Sahoko ; Tsuji, Kohichiro ; Hisakawa, Hiroaki ; Xu, Ming Jiang ; Ebihara, Yasuhiro ; Ishii, Takefumi ; Sugiyama, Daisuke ; Manabe, Atsushi ; Tanaka, Ryuhei ; Ikeda, Yasuo ; Asano, Shigetaka ; Nakahata, Tatsutoshi. / Generation of definitive hematopoietic stem cells from murine early yolk sac and paraaortic splanchnopleures by aorta-gonad-mesonephros region-derived stromal cells. In: Blood. 2001 ; Vol. 98, No. 1. pp. 6-12.
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