Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro

Yeon Suk Jung, Shin Ei Matsumoto, Yoshinori Katakura, Makiko Yamashita, Kosuke Tomimatsu, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Propionibacterium acnes is a gram-positive, non-spore-forming, rod-shaped bacterium that is often detected in normal human skin flora. P. acnes has been associated with many diseases. In this study, we attempted to generate anti-P. acnes human monoclonal antibodies. A phage antibody library was first generated from human peripheral blood mononuclear cells immunized in vitro with P. acnes using the phage display method, and P. acnes-specific phage antibodies were obtained using solid phase panning. Antigen-specific variable region genes were then amplified and recombined into vectors expressing human IgG antibodies. The results indicated that the recombinant human IgG antibodies exhibited P. acnes-specific binding. This study demonstrates that the combined use of an in vitro immunization protocol and the phage display method enables the generation of human monoclonal antibodies against pathogenic bacteria and toxic antigens.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalCytotechnology
Volume57
Issue number2
DOIs
Publication statusPublished - Jun 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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