Generation of human natural killer cells from peripheral blood CD34+ cells mobilized by granulocyte colony-stimulating factor

Katsuto Takenaka, Shin Ichi Mizuno, Mine Harada, Koji Nagafuji, Toshihiro Miyamoto, Hiromi Iwasaki, Tomoaki Fujisaki, Akira Kubota, Yuji Ohno, Fumitou Arima, Hirokazu Shigematsu, Hisashi Gondo, Takashi Okamura, Sehchi Okamura, Shoichi Inaba, Yoshiyuki Niho

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Abstract

We studied the generation of human natural killer (NK) cells from CD34+ cells that were isolated from peripheral blood stem cells (PBSC) mobilized by granulocyte colony-stimulating factor (G-CSF). The isolated CD34+ cells were cultured in the presence of a combination of interleukin-1 (IL-1α), IL-2, and stem cell factor for 5 weeks without marrow stroma. We found that the CD34+ cells isolated from G-CSF-mobilized PBSC (G-CSF/PBSC) could differentiate into a population of NK cells which were CD56+((bright))/CD3- and showed morphologic characteristics of large granular lymphocytes. Immunophenotypic analysis of the NK cells thus generated showed that a small proportion of them expressed CD2, CD8 and CD16 surface markers and approximately half of them coexpressed CD7. This NK population exhibited cytotoxic activity against a NK-sensitive cell line, K562. These observations suggest that CD34+ cells from G-CSF/PBSC contain precursors of NK cells that can differentiate into functional NK cells.

Original languageEnglish
Pages (from-to)788-794
Number of pages7
JournalBritish Journal of Haematology
Volume92
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

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All Science Journal Classification (ASJC) codes

  • Hematology

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