Abstract
Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.
| Original language | English |
|---|---|
| Pages (from-to) | 39-41 |
| Number of pages | 3 |
| Journal | Human Antibodies |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 1991 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
Cite this
Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies. / Shinmoto, Hiroshi; Dosako, Shun'ichi; Tachibana, Hirofumi; Yamada, Koji; Shirahata, Sanetaka; Murakami, Hiroki.
In: Human Antibodies, Vol. 2, No. 1, 01.01.1991, p. 39-41.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies
AU - Shinmoto, Hiroshi
AU - Dosako, Shun'ichi
AU - Tachibana, Hirofumi
AU - Yamada, Koji
AU - Shirahata, Sanetaka
AU - Murakami, Hiroki
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.
AB - Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.
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U2 - 10.3233/HAB-1991-2106
DO - 10.3233/HAB-1991-2106
M3 - Article
C2 - 1873496
AN - SCOPUS:0026030948
VL - 2
SP - 39
EP - 41
JO - Human Antibodies
JF - Human Antibodies
SN - 1093-2607
IS - 1
ER -