Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy