Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies

Hiroshi Shinmoto, Shun'ichi Dosako, Hirofumi Tachibana, Koji Yamada, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two lines of mouse-human hybridomas were fused to produce human bifunctional antibodies. A hybridoma that secretes human IgM (κ) class antiricin monoclonal antibody was treated with actinomycin D to inhibit cell growth. Another cell line, secreting human IgM (λ) class antidiphtheria toxin monoclonal antibody, was 6-thioguanine resistant (HAT sensitive). Six hybridomas secreting IgM with both κ and λ light chains were obtained. Two of them secreted antibodies with antiricin specificity (κ and λ light chains) and antidiphtheria toxin specificity (κ and λ light chains). Antidiphtheria toxin antibodies secreted from other hybrid hybridomas did not contain κ light chain, but λ chain. A hybrid hybridoma clone 5–29 secreted complete hybrid IgM with lowered affinity to diphtheria toxin. These results suggested unbalanced recombination of immunoglobulin polypeptide chains in hybrid hybridomas.

Original languageEnglish
Pages (from-to)39-41
Number of pages3
JournalHuman Antibodies
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

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Hybridomas
Immunoglobulin M
Antibodies
Light
Immunoglobulin Subunits
Monoclonal Antibodies
Bispecific Antibodies
Thioguanine
Diphtheria Toxin
Dactinomycin
Genetic Recombination
Clone Cells
Cell Line
Growth

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies. / Shinmoto, Hiroshi; Dosako, Shun'ichi; Tachibana, Hirofumi; Yamada, Koji; Shirahata, Sanetaka; Murakami, Hiroki.

In: Human Antibodies, Vol. 2, No. 1, 01.01.1991, p. 39-41.

Research output: Contribution to journalArticle

Shinmoto, Hiroshi ; Dosako, Shun'ichi ; Tachibana, Hirofumi ; Yamada, Koji ; Shirahata, Sanetaka ; Murakami, Hiroki. / Generation of hybrid hybridomas secreting human IgM class hybrid antiricin and antidiphtheria toxin antibodies. In: Human Antibodies. 1991 ; Vol. 2, No. 1. pp. 39-41.
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