TY - JOUR
T1 - Generation of Nanog reporter mice that distinguish pluripotent stem cells from unipotent primordial germ cells
AU - Terada, Maiko
AU - Kawamata, Masaki
AU - Kimura, Ryota
AU - Sekiya, Sayaka
AU - Nagamatsu, Go
AU - Hayashi, Katsuhiko
AU - Horisawa, Kenichi
AU - Suzuki, Atsushi
N1 - Funding Information:
The authors thank Drs. Tomoyuki Yamaguchi, Hiromitsu Nakauchi, Masafumi Onodera, and Hiroyuki Miyoshi for sharing reagents and Yuuki Honda, Chiaki Kaieda, Masato Tanaka, and Kanako Motomura for excellent technical assistance. The authors also appreciate the technical assistance from The Research Support Center, Research Center for Human Disease Modeling, Kyushu University Graduate School of Medical Sciences. This work was supported in part by the JSPS KAKENHI (Grant Numbers: JP16H01850, JP16K08592, JP17H05623, JP17K19603, JP18H05102, JP19H01177, and JP19H05267), the Core Research for Evolutional Science and Technology (CREST) Program of the Japan Agency for Medical Research and Development (AMED), the Program for Basic and Clinical Research on Hepatitis of AMED, the Practical Research Project for Rare/Intractable Diseases of AMED, the Research Center Network for Realization of Regenerative Medicine of AMED, the Cooperative Research Project Program of the Medical Institute of Bioregulation in Kyushu University, the Takeda Science Foundation, and the Uehara Memorial Foundation.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Nanog is a core transcription factor specifically expressed not only in the pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs), embryonic germ cells (EGCs), and induced PSCs (iPSCs), but also in the unipotent primordial germ cells (PGCs). Although Nanog promoter/enhancer regions are well characterized by in vitro analyses, direct correlations between the regulatory elements for Nanog expression and in vivo expression patterns of Nanog have not been fully clarified. In this study, we generated Nanog-RFP transgenic (Tg) mice in which expression of red fluorescent protein (RFP) is driven by a 5.2 kb Nanog promoter/enhancer region. As expected, RFP was expressed in the inner cell mass of blastocysts, ESCs, and iPSCs. However, RFP fluorescence was not observed in PGCs, although Nanog was expressed in PGCs. Because RFP fluorescence was visible in the PGC-derived pluripotent EGCs in culture, it was suggested that the reporter gene expression was specifically activated in PSCs. In conclusion, we have generated a novel Nanog-RFP Tg mouse line that can selectively tag PSCs over unipotent PGCs.
AB - Nanog is a core transcription factor specifically expressed not only in the pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs), embryonic germ cells (EGCs), and induced PSCs (iPSCs), but also in the unipotent primordial germ cells (PGCs). Although Nanog promoter/enhancer regions are well characterized by in vitro analyses, direct correlations between the regulatory elements for Nanog expression and in vivo expression patterns of Nanog have not been fully clarified. In this study, we generated Nanog-RFP transgenic (Tg) mice in which expression of red fluorescent protein (RFP) is driven by a 5.2 kb Nanog promoter/enhancer region. As expected, RFP was expressed in the inner cell mass of blastocysts, ESCs, and iPSCs. However, RFP fluorescence was not observed in PGCs, although Nanog was expressed in PGCs. Because RFP fluorescence was visible in the PGC-derived pluripotent EGCs in culture, it was suggested that the reporter gene expression was specifically activated in PSCs. In conclusion, we have generated a novel Nanog-RFP Tg mouse line that can selectively tag PSCs over unipotent PGCs.
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U2 - 10.1002/dvg.23334
DO - 10.1002/dvg.23334
M3 - Article
C2 - 31513343
AN - SCOPUS:85073984082
SN - 1526-954X
VL - 57
JO - Genesis
JF - Genesis
IS - 11-12
M1 - e23334
ER -