Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells

Atsushi Tomokiyo, Kim Hynes, Jia Ng, Danijela Menicanin, Esther Camp, Agnes Arthur, Stan Gronthos, Peter Mark Bartold

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Neural crest cells (NCC) hold great promise for tissue engineering, however the inability to easily obtain large numbers of NCC is a major factor limiting their use in studies of regenerative medicine. Induced pluripotent stem cells (iPSC) are emerging as a novel candidate that could provide an unlimited source of NCC. In the present study, we examined the potential of neural crest tissue-derived periodontal ligament (PDL) iPSC to differentiate into neural crest-like cells (NCLC) relative to iPSC generated from a non-neural crest derived tissue, foreskin fibroblasts (FF). We detected high HNK1 expression during the differentiation of PDL and FF iPSC into NCLC as a marker for enriching for a population of cells with NCC characteristics. We isolated PDL iPSC- and FF iPSC-derived NCLC, which highly expressed HNK1. A high proportion of the HNK1-positive cell populations generated, expressed the MSC markers, whilst very few cells expressed the pluripotency markers or the hematopoietic markers. The PDL and FF HNK1-positive populations gave rise to smooth muscle, neural, glial, osteoblastic and adipocytic like cells and exhibited higher expression of smooth muscle, neural, and glial cell-associated markers than the PDL and FF HNK1-negative populations. Interestingly, the HNK1-positive cells derived from the PDL-iPSC exhibited a greater ability to differentiate into smooth muscle, neural, glial cells and adipocytes, than the HNK1-positive cells derived from the FF-iPSC. Our work suggests that HNK1-enriched NCLC from neural crest tissue-derived iPSC more closely resemble the phenotypic and functional hallmarks of NCC compared to the HNK1-low population and non-neural crest iPSC-derived NCLC. J. Cell. Physiol. 232: 402–416, 2017.

Original languageEnglish
Pages (from-to)402-416
Number of pages15
JournalJournal of cellular physiology
Volume232
Issue number2
DOIs
Publication statusPublished - Feb 1 2017

Fingerprint

Induced Pluripotent Stem Cells
Periodontal Ligament
Neural Crest
Ligaments
Stem cells
Foreskin
Fibroblasts
Cells
Muscle
Neuroglia
Tissue
Population
Smooth Muscle
Regenerative Medicine
Tissue engineering
Tissue Engineering
Adipocytes
Smooth Muscle Myocytes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells. / Tomokiyo, Atsushi; Hynes, Kim; Ng, Jia; Menicanin, Danijela; Camp, Esther; Arthur, Agnes; Gronthos, Stan; Mark Bartold, Peter.

In: Journal of cellular physiology, Vol. 232, No. 2, 01.02.2017, p. 402-416.

Research output: Contribution to journalArticle

Tomokiyo, A, Hynes, K, Ng, J, Menicanin, D, Camp, E, Arthur, A, Gronthos, S & Mark Bartold, P 2017, 'Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells', Journal of cellular physiology, vol. 232, no. 2, pp. 402-416. https://doi.org/10.1002/jcp.25437
Tomokiyo, Atsushi ; Hynes, Kim ; Ng, Jia ; Menicanin, Danijela ; Camp, Esther ; Arthur, Agnes ; Gronthos, Stan ; Mark Bartold, Peter. / Generation of Neural Crest-Like Cells From Human Periodontal Ligament Cell-Derived Induced Pluripotent Stem Cells. In: Journal of cellular physiology. 2017 ; Vol. 232, No. 2. pp. 402-416.
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