Generation of peroxisome-deficient somatic animal cell mutants

Kanji Okumoto, Yukio Fujiki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cell mutants with a genetic defect affecting various cellular phenotypes are widely utilized as a powerful tool in genetic, biochemical, and cell biological research. More than a dozen complementation groups of animal somatic mutant cells defective in peroxisome biogenesis have been successfully isolated in Chinese hamster ovary (CHO) cells and used as a model system reflecting fatal human severe genetic disorders named peroxisome biogenesis disorders (PBD). Isolation and characterization of peroxisome-deficient CHO cell mutants has allowed the identification of PEX genes and the gene products peroxins, which directly leads to the accomplishment of isolation of pathogenic genes responsible for human PBDs, as well as elucidation of their functional roles in peroxisome biogenesis. Here, we describe the procedure to isolate peroxisome-deficient mammalian cell mutants from CHO cells, by making use of an effective, photo-sensitized selection method.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages319-327
Number of pages9
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1595
ISSN (Print)1064-3745

Fingerprint

Peroxisomes
Cricetulus
Ovary
Genes
Inborn Genetic Diseases
Medical Genetics
Molecular Biology
Phenotype
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Okumoto, K., & Fujiki, Y. (2017). Generation of peroxisome-deficient somatic animal cell mutants. In Methods in Molecular Biology (pp. 319-327). (Methods in Molecular Biology; Vol. 1595). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6937-1_29

Generation of peroxisome-deficient somatic animal cell mutants. / Okumoto, Kanji; Fujiki, Yukio.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 319-327 (Methods in Molecular Biology; Vol. 1595).

Research output: Chapter in Book/Report/Conference proceedingChapter

Okumoto, K & Fujiki, Y 2017, Generation of peroxisome-deficient somatic animal cell mutants. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1595, Humana Press Inc., pp. 319-327. https://doi.org/10.1007/978-1-4939-6937-1_29
Okumoto K, Fujiki Y. Generation of peroxisome-deficient somatic animal cell mutants. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 319-327. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6937-1_29
Okumoto, Kanji ; Fujiki, Yukio. / Generation of peroxisome-deficient somatic animal cell mutants. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 319-327 (Methods in Molecular Biology).
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