Generation of rat monoclonal antibodies specific for Ad4BPSF-1

Chikako Yokoyama; Tomoko Komatsu; Hidesato Ogawa; Ken Ichirou Morohashi; Masayuki Azuma; Taro Tachibana

doi:10.1089/hyb.2008.0084

Generation of rat monoclonal antibodies specific for Ad4BPSF-1

Chikako Yokoyama, Tomoko Komatsu, Hidesato Ogawa, Ken Ichirou Morohashi, Masayuki Azuma, Taro Tachibana

Biology of Sex Difference

Research output: Contribution to journal › Article

9 Citations (Scopus)

Abstract

Ad4BP/SF-1 (adrenal4 binding protein/steroidogenic factor-1[NR5A1]) is an essential nuclear receptor required for animal reproduction and endocrine regulation. The present study reports on monoclonal antibodies (MAbs) directed against mouse Ad4BP/SF-1, which were produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The produced MAbs reacted with both recombinant and endogenous Ad4BP/SF-1. These MAbs will be useful in immunolocalization and immunoblotting experiments conducted on different tissue types to determine the levels of expression of Ad4BP/SF-1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Hybridoma
Volume	28
Issue number	2
DOIs	https://doi.org/10.1089/hyb.2008.0084
Publication status	Published - Apr 1 2009

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K. I., Azuma, M., & Tachibana, T. (2009). Generation of rat monoclonal antibodies specific for Ad4BPSF-1. Hybridoma, 28(2), 113-119. https://doi.org/10.1089/hyb.2008.0084

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abstract = "Ad4BP/SF-1 (adrenal4 binding protein/steroidogenic factor-1[NR5A1]) is an essential nuclear receptor required for animal reproduction and endocrine regulation. The present study reports on monoclonal antibodies (MAbs) directed against mouse Ad4BP/SF-1, which were produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The produced MAbs reacted with both recombinant and endogenous Ad4BP/SF-1. These MAbs will be useful in immunolocalization and immunoblotting experiments conducted on different tissue types to determine the levels of expression of Ad4BP/SF-1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.",

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AU - Tachibana, Taro

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