Generation of rat monoclonal antibodies specific for Ad4BPSF-1

Chikako Yokoyama, Tomoko Komatsu, Hidesato Ogawa, Ken Ichirou Morohashi, Masayuki Azuma, Taro Tachibana

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ad4BP/SF-1 (adrenal4 binding protein/steroidogenic factor-1[NR5A1]) is an essential nuclear receptor required for animal reproduction and endocrine regulation. The present study reports on monoclonal antibodies (MAbs) directed against mouse Ad4BP/SF-1, which were produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The produced MAbs reacted with both recombinant and endogenous Ad4BP/SF-1. These MAbs will be useful in immunolocalization and immunoblotting experiments conducted on different tissue types to determine the levels of expression of Ad4BP/SF-1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalHybridoma
Volume28
Issue number2
DOIs
Publication statusPublished - Apr 1 2009

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Generation of rat monoclonal antibodies specific for Ad4BPSF-1'. Together they form a unique fingerprint.

  • Cite this

    Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K. I., Azuma, M., & Tachibana, T. (2009). Generation of rat monoclonal antibodies specific for Ad4BPSF-1. Hybridoma, 28(2), 113-119. https://doi.org/10.1089/hyb.2008.0084