Generation of transgenic mice producing a human transthyretin variant: A possible mouse model for familial amyloidotic polyneuropathy

Hiroyuki Sasaki, Shigenobu Toné, Masamitsu Nakazato, Katsuji Yoshioka, Hisayuki Matsuo, Yoshihiro Kato, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Type I familial amyloidotic polyneuropathy(FAP) results from the systemic deposition of a plasma transthyretin(TTR) variant with a Val ▶ Met change at position 30. In an attempt to establish a model of this disease, we generated transgenic mice producing the variant TIR. A DNA fragment containing the mouse metallothionein-I promoter fused to the structural gene coding for the human TTR variant was microinjected into fertilized mouse eggs. Among 72 mice that developed from these eggs, ten carried the fusion gene and three of these showed significant concentrations of the variant TTR in their serum. These mice may be useful in elucidating the pathogenesis of FAP and in establishing a therapy for this intractable disorder.

Original languageEnglish
Pages (from-to)794-799
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number2
DOIs
Publication statusPublished - Sep 16 1986

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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