Generation of virus-specific CD8 + T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp

Seisuke Tajimi, Masakazu Kondo, Teruyuki Nakanishi, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto

Research output: Contribution to journalArticle

Abstract

Although a previous study using ginbuna crucian carp suggested that cell-mediated immunity can be induced by the oral administration of inactivated viruses, which are exogenous antigens, there is no direct evidence that CD8 + cytotoxic T cells (CTLs) in teleost fish are generated by vaccination with exogenous antigens. In the present study, we investigated whether antigen-specific CD8 + CTLs in ginbuna crucian carp can be elicited by intestinal immunization with an exogenous antigen without any adjuvant. The IFNγ-1 and T-bet mRNA expressions were up-regulated in intestinal leukocytes following the administration of formalin-inactivated crucian hematopoietic necrosis virus (FI-CHNV), whereas the down-regulation of these genes was observed in kidney leukocytes. Furthermore, an increase in the percentage of proliferating CD8 + cells was detected in the posterior portion of the hindgut, suggesting that the virus-specific CTLs are locally generated in this site. In addition, cell-mediated cytotoxicity against CHNV-infected syngeneic cells and the in vivo inhibition of viral replication were induced by immunization with FI-CHNV. Unexpectedly, intraperitoneal immunization with FI-CHNV induced a type I helper T cell (Th1)-response in the intestine, but not in the kidney; however, its effect was slightly lower than that reported after intestinal immunization. These findings suggest that the posterior portion of the intestine is an important site for generating virus-specific CTLs by vaccination with the inactivated vaccine.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume93
DOIs
Publication statusPublished - Apr 1 2019

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Carps
Intestines
Vaccination
Viruses
T-Lymphocytes
Immunization
Formaldehyde
Necrosis
Antigens
Leukocytes
CD8 Antigens
Kidney
Inactivated Vaccines
Helper-Inducer T-Lymphocytes
Cellular Immunity
Oral Administration
Fishes
Down-Regulation
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Generation of virus-specific CD8 + T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp . / Tajimi, Seisuke; Kondo, Masakazu; Nakanishi, Teruyuki; Nagasawa, Takahiro; Nakao, Miki; Somamoto, Tomonori.

In: Developmental and Comparative Immunology, Vol. 93, 01.04.2019, p. 37-44.

Research output: Contribution to journalArticle

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