Genes encoding small heat shock proteins of the silkworm, Bombyx mori

Daisuke Sakano, Bin Li, Qingyou Xia, Kohji Yamamoto, Hiroshi Fujii, Yoichi Aso

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Small heat shock protein (sHSP) is a family of ubiquitous polypeptides involved in a variety of physiological phenomena. From the silkworm, Bombyx mori, we isolated and sequenced the following cDNAs encoding sHSPs: shsp19.9, shsp20.1, shsp20.4, shsp20.8, shsp21.4, and shsp23.7. shsp21.4 was nearly twice as large in size as other shsps. The deduced amino acid sequence of sHSP21.4 was similar to that of Drosophila melanogaster CG14207-PA. Other sHSPs were highly similar to each other and, in a phylogenetic tree, formed a cluster including Plodia interpunctella αCP25. It was speculated that shsp21.4 has at least one intron in genome while other shsps do not. The transcripts of all shsps were subtle, but were constitutively detected in various tissues. Heat shock triggered a substantial increase in the transcripts other than shsp21.4. The B. mori sHSPs are perhaps classified into at least two groups: sHSP21.4 and others.

Original languageEnglish
Pages (from-to)2443-2450
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number10
DOIs
Publication statusPublished - Nov 6 2006

Fingerprint

Small Heat-Shock Proteins
Gene encoding
Bombyx
Introns
Complementary DNA
Genes
Tissue
Amino Acids
Peptides
Physiological Phenomena
Drosophila melanogaster
Amino Acid Sequence
Shock
Hot Temperature
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Genes encoding small heat shock proteins of the silkworm, Bombyx mori. / Sakano, Daisuke; Li, Bin; Xia, Qingyou; Yamamoto, Kohji; Fujii, Hiroshi; Aso, Yoichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 10, 06.11.2006, p. 2443-2450.

Research output: Contribution to journalArticle

Sakano, Daisuke ; Li, Bin ; Xia, Qingyou ; Yamamoto, Kohji ; Fujii, Hiroshi ; Aso, Yoichi. / Genes encoding small heat shock proteins of the silkworm, Bombyx mori. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 10. pp. 2443-2450.
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