Genes essential for the morphogenesis of the Shiga toxin 2-transducing phage from Escherichia coli O157:H7

Shakhinur Islam Mondal, Md Rakibul Islam, Akira Sawaguchi, Md Asadulghani, Tadasuke Ooka, Yasuhiro Gotoh, Yasuhiro Kasahara, Yoshitoshi Ogura, Tetsuya Hayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Shiga toxin 2 (Stx2), one of the most important virulence factors of enterohaemorrhagic Escherichia coli (EHEC), is encoded by phages. These phages (Stx2 phages) are often called lambda-like. However, most Stx2 phages are short-tailed, thus belonging to the family Podoviridae, and the functions of many genes, especially those in the late region, are unknown. In this study, we performed a systematic genetic and morphological analysis of genes with unknown functions in Sp5, the Stx2 phage from EHEC O157:H7 strain Sakai. We identified nine essential genes, which, together with the terminase genes, determine Sp5 morphogenesis. Four of these genes most likely encoded portal, major capsid, scaffolding and tail fiber proteins. Although exact roles/functions of the other five genes are unknown, one was involved in head formation and four were required for tail formation. One of the four tail genes encoded an unusually large protein of 2,793 amino-acid residues. Two genes that are likely required to maintain the lysogenic state were also identified. Because the late regions of Stx2 phages from various origins are highly conserved, the present study provides an important basis for better understanding the biology of this unique and medically important group of bacteriophages.

Original languageEnglish
Article number39036
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Dec 14 2016

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Shiga Toxin 2
Escherichia coli O157
Essential Genes
Morphogenesis
Bacteriophages
Genes
Enterohemorrhagic Escherichia coli
Podoviridae
Capsid
Virulence Factors
Proteins
Head
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

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Genes essential for the morphogenesis of the Shiga toxin 2-transducing phage from Escherichia coli O157:H7. / Mondal, Shakhinur Islam; Islam, Md Rakibul; Sawaguchi, Akira; Asadulghani, Md; Ooka, Tadasuke; Gotoh, Yasuhiro; Kasahara, Yasuhiro; Ogura, Yoshitoshi; Hayashi, Tetsuya.

In: Scientific reports, Vol. 6, 39036, 14.12.2016.

Research output: Contribution to journalArticle

Mondal, Shakhinur Islam ; Islam, Md Rakibul ; Sawaguchi, Akira ; Asadulghani, Md ; Ooka, Tadasuke ; Gotoh, Yasuhiro ; Kasahara, Yasuhiro ; Ogura, Yoshitoshi ; Hayashi, Tetsuya. / Genes essential for the morphogenesis of the Shiga toxin 2-transducing phage from Escherichia coli O157:H7. In: Scientific reports. 2016 ; Vol. 6.
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