Genetic analysis of plasmid-specific pheromone signaling encoded by ppd1 in enterococcus faecalis

Jiro Nakayama; Akinori Suzuki

doi:10.1271/bbb.61.1796

Genetic analysis of plasmid-specific pheromone signaling encoded by ppd1 in enterococcus faecalis

Jiro Nakayama, Akinori Suzuki

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific.

Original language	English
Pages (from-to)	1796-1799
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	11
DOIs	https://doi.org/10.1271/bbb.61.1796
Publication status	Published - Jan 1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Genetic analysis of plasmid-specific pheromone signaling encoded by ppd1 in enterococcus faecalis",

abstract = "Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific.",

author = "Jiro Nakayama and Akinori Suzuki",

note = "Funding Information: Acknovvledgments. We thank D. B. Clewell for providing us E. faecalis strains. We also thank D. B. Clewell and M. Yamanouchi for critical reading of this paper. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 08760112) from the Ministry of Education, Science, and Culture of Japan, by a grant from Nissan Science Foundation, by a grant for The Naito Foundation, and by a grant from Nougeikagaku Shoureikai.",

year = "1997",

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doi = "10.1271/bbb.61.1796",

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AU - Suzuki, Akinori

N1 - Funding Information: Acknovvledgments. We thank D. B. Clewell for providing us E. faecalis strains. We also thank D. B. Clewell and M. Yamanouchi for critical reading of this paper. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 08760112) from the Ministry of Education, Science, and Culture of Japan, by a grant from Nissan Science Foundation, by a grant for The Naito Foundation, and by a grant from Nougeikagaku Shoureikai.

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N2 - Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific.

AB - Certain plasmids in Enterococcus faecalis encode a mating response to recipient-produced peptide sex pheromones. Targeted disruption of tra genes on pPD1 suggested that TraA plays a central role in the plasmid-specific pheromone signaling pathway. TraA functioned as a negative regulator for the pheromone-inducible conjugal transfer. Complementation analysis of pPD1 tra gene mutants by pAD1 suggested that the pheromone binding function of TraC was non-specific between these plasmids, but the function of TraA and the pheromone shutdown function of TraB are plasmid-specific.

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Genetic analysis of plasmid-specific pheromone signaling encoded by ppd1 in enterococcus faecalis

Abstract

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